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Record W4412124417 · doi:10.1126/sciadv.adu4909

Spatiotemporal reconstruction of the North American A(H5N1) outbreak reveals successive lineage replacements by descendant reassortants

2025· article· en· W4412124417 on OpenAlexaffabout
Anthony V. Signore, Jolene A. Giacinti, Megan Jones, Cassidy N. G. Erdelyan, Angela McLaughlin, Tamiru N. Alkie, Sherri Cox, Stéphane Lair, Claire M. Jardine, Brian Stevens, Maria Bravo-Araya, Neil Pople, Margo J. Pybus, Tamiko Hisanaga, Wanhong Xu, Janice Koziuk, Oliver Lung, Peter Kruczkiewicz, Mathew Fisher, Jordan Wight, Ishraq Rahman, Kathryn E. Hargan, Andrew S. Lang, Orie Hochman, Davor Ojkić, Carmencita Yason, Laura Bourque, Trent K. Bollinger, Jennifer F. Provencher, Sarah Ogilvie, Amanda Clark, Robyn MacPhee, Hazel Eaglesome, Sayrah Gilbert, Kelsey Saboraki, Richard Davis, Alexandra Jerao, Matthew Ginn, Catherine Soos, Yohannes Berhane

Bibliographic record

VenueScience Advances · 2025
Typearticle
Languageen
FieldMedicine
TopicInfluenza Virus Research Studies
Canadian institutionsUniversity of ManitobaAgriculture Food and Rural DevelopmentMinistry of Natural Resources and ForestryUniversity of Prince Edward IslandMemorial University of NewfoundlandCanadian Food Inspection AgencyUniversity of SaskatchewanUniversity of AlbertaAlberta Environment and Protected AreasShared HealthEnvironment and Climate Change CanadaUniversity of British ColumbiaCegep de Saint HyacintheUniversity of Guelph
Fundersnot available
KeywordsInfluenza A virus subtype H5N1OutbreakCladeReassortmentBiologyHighly pathogenicLineage (genetic)PhylogeneticsVirologyInfluenza A virusEvolutionary biologyGeneticsVirusInfectious disease (medical specialty)GeneCoronavirus disease 2019 (COVID-19)Medicine

Abstract

fetched live from OpenAlex

The November 2021 introduction of highly pathogenic avian influenza A(H5N1) clade 2.3.4.4b into North America triggered a devastating outbreak, affecting more than 180 million domestic birds and spreading to more than 80 wildlife species across Canada and the US. From this outbreak, we have sequenced 2955 complete A(H5N1) viral genomes from samples collected in Canada and, in conjunction with previously published data, performed multifaceted phylodynamic analyses. These analyses reveal extensive diversification of A(H5N1) viruses via reassortment with low-pathogenic avian influenza viruses. We find evidence of repeated ancestral strain replacement by direct descendants, indicative of compounding viral fitness increases. Spatiotemporal modeling identified critical geographic areas facilitating transcontinental spread and demonstrated genotype-specific host dynamics, offering essential data for ongoing control and prevention strategies.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.001
metaresearch head score (Gemma)0.002
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesScience and technology studies
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Observational · Consensus signal: Observational
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.205
Threshold uncertainty score1.000

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0010.002
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.002
Science and technology studies0.0000.003
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.024
GPT teacher head0.374
Teacher spread0.349 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one teacher head, not a consensus.

Study designObservational
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations23
Published2025
Admission routes2
Has abstractyes

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