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Record W4412474498 · doi:10.1177/10406387251355254

An investigation of the role of wild rats in transmitting <i>Leptospira</i> spp. to stray cats and dogs in Malaysia

2025· article· en· W4412474498 on OpenAlexaff
Zafirah Muhd, Kuan Hua Khor, Abdul Rahman Alashraf, Seng Fong Lau, Rozanaliza Radzi, Siti Khairani Bejo, Rohaidah Hashim

Bibliographic record

VenueJournal of Veterinary Diagnostic Investigation · 2025
Typearticle
Languageen
FieldImmunology and Microbiology
TopicLeptospirosis research and findings
Canadian institutionsUniversity of Guelph
FundersUniversiti Putra Malaysia
KeywordsLeptospiraLeptospirosisLeptospira interrogansDirect agglutination testSerotypeCATSBiologyVeterinary medicineMicrobiologyVirologyAntibodySerologyMedicineImmunologyInternal medicine

Abstract

fetched live from OpenAlex

Stray cats and dogs have been reported to shed Leptospira spp., and wild rats are speculated to be involved. We aimed to elucidate the role of wild rats in transmitting Leptospira to stray cats and dogs in Malaysia. We tested sera from 124 wild rats with the microscopic agglutination test (MAT): 88 of 122 (72%) sera were positive (titer ≥1:100), with the predominant serovars Icterohaemorrhagiae, Bataviae, Ballum, Javanica, Lai, and Pomona. With a Leptospira -specific PCR assay, we detected pathogenic Leptospira spp. in 33 of 124 (27%) kidney samples and 13 of 79 (16%) urine samples. Isolates obtained by culture of rat kidney and urine were identified to the species level with MAT using hyperimmune sera and the PCR assay. From 29 isolates, 2 pathogenic species were identified: L. interrogans serovar Bataviae and L. borgpetersenii serovar Javanica. Phylogenetic analysis using partial 16S rDNA sequences of the Leptospira spp. from the wild rats indicated that the species were similar to isolates from stray cats and dogs in previous studies. We confirmed that wild rats carried pathogenic Leptospira spp. and were a potential source of leptospiral infection of stray cats and dogs in Malaysia.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Observational · Consensus signal: Observational
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.008
Threshold uncertainty score0.016

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0010.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.012
GPT teacher head0.260
Teacher spread0.247 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designObservational
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations1
Published2025
Admission routes1
Has abstractyes

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