Culture conditions of symbiotic fungus <i>Coprinellus radians</i> and its effects on seedlings of <i>Cremastra appendiculata</i> (D.Don.) Makino (Orchidaceae)
Bibliographic record
Abstract
This study screened and optimized the formulations and culture conditions of strain at all levels of Coprinellus radians; building on this foundation, the symbiotic seedling cultivation of Coprinellus radians with Cremastra appendiculata was optimized to explore the best conditions. The results revealed that the suitable conditions for the cultivation of the parental strain of Coprinellus radians were PDA as the basal medium with the addition of 10 g·L−1 fructose, 5·g·L−1 yeast extract, and 15 g·L−1 bran, and the colony diameter was 83.90 ± 0.82 mm and the dry weight of the hyphae was 2.4 ± 0.1 mg under these conditions. Further studies show that the colony diameter of the parental strain of Coprinellus radians was 84.77 ± 1.42 mm and the dry weight of mycelium was 2.5 ± 0.1 mg at 35 °C, pH = 6.0, and dark incubation. Subsequently, the formulation suitable for the cultivation of the primary and cultivation strain was a mixture of willow wood, cottonseed hulls, and sweet potato flour in a mass ratio of 8:1:1 with a water content of 30%. The symbiotic seedling cultivation methods were feasible, the suitable conditions for the symbiotic cultivation of Cremastra appendiculata with Coprinellus radians were: 5 kg·m−2 of wood block dosage, 50 mg·m−2 of seed dosage, and 0.5 kg·m−2 of cultivation strain dosage, under which the germination rate of Cremastra appendiculata was 33.69 ± 1.71%, and the emergence rate was 2.81 ± 0.36%. Results of these studies have screened suitable culture conditions for the strain of Coprinellus radians and conducted preliminary experiments for the establishment of symbiotic seedling propagation technology for Cremastra appendiculata.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.001 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".