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Interactive effects of copper contamination and salinization across multiple genotypes of Daphnia magna

2025· preprint· en· W4412634587 on OpenAlexaff
Andrea Michelle Hernandez Villatoro, Jeremy J. Piggott, Adam P. Ryan, Pepijn Luijck, Charlotte Carrier‐Belleau

Bibliographic record

Venuenot available
Typepreprint
Languageen
FieldEnvironmental Science
TopicHeavy metals in environment
Canadian institutionsTrinity College
Fundersnot available
KeywordsDaphnia magnaContaminationCopperEnvironmental scienceDaphniaGenotypeWater contaminationBiologyEcologyChemistry

Abstract

fetched live from OpenAlex

Understanding how organisms respond to multiple environmental stressors is essential for predicting ecosystem impacts in the face of increasing anthropogenic pressures. However, few studies have explicitly examined how genotypes of the same species respond to combined stressors, with the specific objective of disentangling variation both within and across geographic locations. In this study, we examined the individual and combined effects of copper contamination and elevated salinity on multiple genotypes of Daphnia magna from U.S. and French populations. Our findings revealed that copper exposure consistently increased mortality across all genotypes, with U.S. genotypes displaying greater sensitivity than French counterparts. Salinity stress primarily reduced fecundity, and again, U.S. genotypes exhibited lower resilience. Under combined copper and salinity stress, however, U.S. genotypes showed survival benefits, suggesting potential cross-tolerance mechanisms between these stressors. Moreover, there was substantial variation in the response to both stressors within both locations. This genotype-specific variation underscores the necessity of considering genetic factors and genotype-specific sensitivity/tolerance into ecosystem management and conservation strategies, particularly under multiple-stressor scenarios. Further exploration of the genetic pathways and adaptation potential driving these responses will enhance our ability to support biodiversity and ecosystem resilience amid global environmental change.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.011
Threshold uncertainty score0.021

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.006
GPT teacher head0.267
Teacher spread0.261 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2025
Admission routes1
Has abstractyes

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