Multifunctional bioactivities of compounds derived from Streptomyces sp. MFB28: Antibacterial, antioxidant, and antiproliferative effects
Bibliographic record
Abstract
The growing threat of antimicrobial resistance and limited cancer treatment options necessitate novel bioactive compounds. This study evaluated the antibacterial, antioxidant, and antiproliferative activities of Streptomyces sp. MFB28, which was isolated from Moroccan soil. Secondary metabolites were extracted from culture supernatant and mycelium using chloroform, ethanol, and water. Antibacterial activity was tested against five bacterial strains by disk diffusion and microdilution assays; antioxidant capacity was assessed via DPPH scavenging; cytotoxicity and apoptosis were evaluated using MTT assays on P3 and U2OS cells, and flow cytometry on U2OS cells. The chloroform extract exhibited potent antibacterial activity against Gram-positive bacteria (MICs: 6.25 µg/mL for S. aureus , 3.125 µg/mL for Micrococcus luteus ). Antioxidant analysis revealed strong radical scavenging activity in both aqueous and chloroform extracts, with IC₅₀ = 60.1 and 133.3 µg/mL, respectively, while the ethanol extract showed the most potent effect (IC₅₀ = 54.3 µg/mL). In cytotoxicity assays, the chloroform and ethanol extracts showed significant antiproliferative effects on murine myeloid P3 cells, with IC₅₀ = 10 and 18.587 µg/mL, respectively. Both induced dose-dependent cancer cell growth inhibition, with complete inhibition at 40 µg/mL. Flow cytometry on U2OS cells revealed apoptosis induction via sub-G1 arrest (chloroform extract) and G1/S arrest (aqueous extract), indicating distinct mechanisms of action. These findings highlight the therapeutic potential of Streptomyces sp. MFB28, particularly for its selective antibacterial effects and dual antioxidant–anticancer properties. The strain’s origin from Moroccan soil underscores the untapped diversity of actinobacteria in this environment, offering a promising source of novel natural compounds. This study lays the groundwork for future chemical characterization and in vivo evaluation of its bioactive metabolites for potential drug development. • Streptomyces sp. MFB28 shows multiple potent bioactivities. • Chloroform extract is active mainly against Gram-positive bacteria. • Ethanolic extract has the lowest IC50 in antioxidant assays. • Chloroform and ethanol extracts inhibit U2OS and P3 cell proliferation. • Extracts induce apoptosis in U2OS cells by flow cytometry.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".