Early-life vitamin A supplementation modulates the skeletal muscle transcriptome and intramuscular fat deposition in feedlot-finished beef steers
Bibliographic record
Abstract
This study evaluated the effects of neonatal vitamin A supplementation on intramuscular fat (IMF) deposition and the molecular mechanisms regulating adipogenesis and lipogenesis in Montana × Nellore crossbred beef steers. We hypothesize that neonatal vitamin A supplementation induces long-term transcriptional changes associated with IMF development. Thirty-four calves were randomly assigned to two groups: a control receiving a placebo and a treatment group receiving a single intramuscular injection of 300,000 IU of vitamin A at birth. Longissimus thoracis (LT) muscle samples were collected via biopsy at 40 days of age (cow-calf phase) and again at 400 ± 10 days of age (finishing phase) immediately after slaughter. At deboning (48 hours postmortem), LT samples were collected between the 12th and 13th ribs for chemical composition assessment. Vitamin A supplementation had no significant effect on weaning weight, average daily gain, final body weight, backfat thickness, or ribeye area (P > 0.05). However, IMF content was significantly higher (P < 0.01) in the vitamin A-supplemented (4.10%) compared to control (2.57%). Transcriptomic analysis revealed 52 and 165 differentially expressed genes (DEGs; FDR < 0.05) during the cow-calf and finishing phases, respectively. Functional enrichment analyses showed that vitamin A supplementation upregulated the actin cytoskeleton, insulin, and calcium signaling pathways during the cow-calf phase, and the PI3K-Akt, AMPK, and PPAR signaling pathways during the finishing phase. Notably, key genes associated with adipogenesis and lipogenesis, such as KRAS, ITGB6, CALML6, CISH, and ATF3 in the cow-calf phase, and LEP, ACACA, FASN, SCD, ELOVL6, and CIDEC in the finishing phase, were upregulated in response to vitamin A, as well as several adipogenic regulators, including PPARG, C/EBPA, and FABP4. Pathway enrichment analysis highlighted the PI3K-Akt signaling pathway as a central mediator of vitamin A's effects on adipocyte differentiation and lipid metabolism. Thus, neonatal vitamin A supplementation can enhance IMF deposition in beef steers by modulating key molecular pathways, without negatively impacting growth performance or carcass traits.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".