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Record W4413095618 · doi:10.1016/j.immuno.2025.100056

Challenges and future directions of AIRR-seq-based diagnostics

2025· article· en· W4413095618 on OpenAlexaff
Ulrik Stervbo, Paraskevas Filippidis, Felix Breden, Lindsay G. Cowell, Frédéric Davi, Victor Greiff, Anton W. Langerak, Eline T. Luning Prak, Alexandra F. Sharland, Enkelejda Miho, Pieter Meysman

Bibliographic record

VenueImmunoInformatics · 2025
Typearticle
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicMolecular Biology Techniques and Applications
Canadian institutionsSimon Fraser University
FundersNational Institute of Allergy and Infectious DiseasesNational Health and Medical Research CouncilNational Institutes of HealthSchweizerischer Nationalfonds zur Förderung der Wissenschaftlichen ForschungDeutsche Forschungsgemeinschaft
KeywordsComputer science

Abstract

fetched live from OpenAlex

Adaptive Immune Receptor Repertoire sequencing (AIRR-seq) is a promising diagnostic method across various clinical conditions, yet its widespread implementation faces several challenges. This perspective examines the current landscape of AIRR-seq diagnostics and outlines key obstacles and opportunities for advancement. Critical challenges include the need for standardized quality controls, privacy protection under General Data Protection Regulation (GDPR) and Health Insurance Portability and Accountability Act (HIPAA) frameworks, and the development of clinically compatible bioinformatics pipelines. Machine learning approaches offer potential solutions for interpreting complex repertoire signatures, though these models must balance accuracy with interpretability for clinical adoption. Future applications may include early disease detection, prognosis, and monitoring of treatment and vaccine responses. However, successful clinical integration will require sustained collaboration among funding bodies, regulatory agencies, researchers, diagnosticians, and clinicians to establish clear guidelines and expand existing repositories with well-characterized patient samples. The collaborative efforts of the AIRR Diagnostics Working Group and the AIRR Community's initiatives are working towards unlocking the potential of AIRR-seq in precision medicine and enhancing diagnostic capabilities.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.086
metaresearch head score (Gemma)0.049
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Not applicable · Consensus signal: none
GenreCandidate signal: Review · Consensus signal: none
Teacher disagreement score0.086
Threshold uncertainty score0.455

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0860.049
Meta-epidemiology (narrow)0.0020.001
Meta-epidemiology (broad)0.0020.002
Bibliometrics0.0020.002
Science and technology studies0.0020.008
Scholarly communication0.0090.016
Open science0.0070.007
Research integrity0.0120.010
Insufficient payload (model declined to judge)0.0120.005

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.007
GPT teacher head0.258
Teacher spread0.252 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designNot applicable
Domainnot available
GenreReview

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations2
Published2025
Admission routes1
Has abstractyes

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