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Record W4413128315 · doi:10.1101/2025.08.11.669729

Alternative splicing of NUMB correlates with tumor immune evasion and metabolic adaptation

2025· preprint· en· W4413128315 on OpenAlexaff
Yangjing Zhang, C. Jane McGlade

Bibliographic record

VenuebioRxiv (Cold Spring Harbor Laboratory) · 2025
Typepreprint
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicRNA Research and Splicing
Canadian institutionsSickKids FoundationHospital for Sick Children
Fundersnot available
KeywordsEvasion (ethics)NUMBImmune systemAdaptation (eye)Alternative splicingRNA splicingBiologyComputational biologyNeuroscienceCell biologyGeneticsGeneRNAExon

Abstract

fetched live from OpenAlex

Abstract Alternative splicing of the NUMB gene at exon 9 generates protein isoforms linked to tumor growth and metastasis, but its impact on the tumor immune microenvironment remains unclear. Using RNA sequencing data from over 5,000 tumors across 16 cancer types, including breast cancer subtypes, we found that tumors with high NUMB exon 9 inclusion exhibit lower expression of immune-related genes, reduced immune cell infiltration, and decreased cytolytic activity, consistent with an immune “cold” phenotype. This pattern was consistent across multiple cancer types and breast cancer subtypes. Additionally, exon 9-high tumors showed evidence of increased oxidative phosphorylation, suggesting metabolic adaptations that support tumor progression. These findings identify NUMB exon 9 inclusion as a potential biomarker of immune evasion and highlight opportunities for patient stratification and targeted therapies based on NUMB exon 9 inclusion levels.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.001
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.002
Threshold uncertainty score0.006

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.001
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0020.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.010
GPT teacher head0.233
Teacher spread0.223 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations1
Published2025
Admission routes1
Has abstractyes

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