MétaCan
Menu
Back to cohort
Record W4413197164 · doi:10.1101/2025.08.08.669192

Procrustean pseudo-landmark methods in Python to measure massive quantities of leaf shape data

2025· preprint· en· W4413197164 on OpenAlexaff
Asia T. Hightower, Ricardo A. Urquidi Camacho, Alexandra Papamichail, Evan Adamski, Claudia Colligan, Aidan Deneen, Georgia Dunn, Justine Haziza, C. Noel Henley, Arnan Pawawongsak, Sean Ward, Manica Balant, Christopher B. Blackwood, Charles H. Cannon, Andrea L. Case, Aman Y. Husbands, Emily B. Josephs, Zoë Migicovsky, Rachel P. Naegele, Eric Patterson, Yenny Alejandra Saavedra-Rojas, Daniel H. Chitwood

Bibliographic record

VenuebioRxiv (Cold Spring Harbor Laboratory) · 2025
Typepreprint
Languageen
FieldAgricultural and Biological Sciences
TopicLeaf Properties and Growth Measurement
Canadian institutionsAcadia University
FundersNational Institutes of HealthNational Science Foundation
KeywordsShape analysis (program analysis)Procrustes analysisLandmarkPython (programming language)PhyllotaxisPrincipal component analysisMathematicsArtificial intelligencePattern recognition (psychology)Computer scienceBiologyBotanyEcology

Abstract

fetched live from OpenAlex

Premise: When examining leaf shapes that are different from one another, it can be difficult to compare both the overall leaf shape and points along the leaf margin in biologically and statistically meaningful ways. Methods: To address this problem, we present a simple and user-friendly leaf shape analysis method in Jupyter Notebook and Python that uses pseudo-landmarks and generalized Procrustes analysis to measure and compare the shape of any leaf. To demonstrate our analysis, we created a repository of real leaves gathered from eight experimental datasets. Results: Using our leaf repository, we explain how we can use pseudo-landmarks to compare all leaf shapes, both within and between species, using dimension reduction techniques like principal component analysis and can predict leaf shapes using pseudo-landmarks through linear discriminant analysis. Our leaf shape analysis also maps differences in shape as leaves grew around a rosette, showing the transition of shape across development (phyllotaxy). Finally, we showed how the relationship between leaf shape variation and genetic diversity can be investigated by combining shape with genetic data. Discussion: Through the use of generalized Procrustes analysis and pseudo-landmarks, our leaf shape analysis presents a powerful tool for examining the shape of any leaf across multiple biological, ecological, evolutionary, and developmental scales.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.002
metaresearch head score (Gemma)0.001
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesMeta-epidemiology (narrow)
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.439
Threshold uncertainty score1.000

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0020.001
Meta-epidemiology (narrow)0.0010.000
Meta-epidemiology (broad)0.0010.000
Bibliometrics0.0000.001
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0020.002
Research integrity0.0000.001
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.083
GPT teacher head0.286
Teacher spread0.203 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one teacher head, not a consensus.

Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations1
Published2025
Admission routes1
Has abstractyes

Explore more

Same venuebioRxiv (Cold Spring Harbor Laboratory)Same topicLeaf Properties and Growth MeasurementFrench-language works237,207