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Record W4413372772 · doi:10.1101/2025.08.15.670587

Floral microbes provisioned by <i>Osmia lignaria</i> establish in larval food stores, but do not affect bee development or survival

2025· preprint· en· W4413372772 on OpenAlexfundno aff
A.N. Martin, Clara Stuligross, Neal M. Williams, Helen M. Noroian, Rachel L. Vannette

Bibliographic record

VenuebioRxiv (Cold Spring Harbor Laboratory) · 2025
Typepreprint
Languageen
FieldAgricultural and Biological Sciences
TopicPlant and animal studies
Canadian institutionsnot available
FundersKaren C. Drayer Wildlife Health CenterUniversity of California, DavisDalhousie UniversityNational Science Foundation
KeywordsLarvaProvisioningBiologyAffect (linguistics)EcologyZoologyCommunicationEngineeringPsychology

Abstract

fetched live from OpenAlex

Abstract Microbial dispersal and subsequent establishment among linked habitats can be used to examine drivers of community assembly and function. Flowers host microbial communities that can be acquired and vectored by bees to new flowers, establish within the adult bee gut, and enter food stores (e.g., pollen provisions) of developing larvae. Yet, whether microbes vectored by insects or applied for biocontrol can establish across these habitats and if they affect bee fitness remain unknown. Here, we applied microbes to flowers visited by blue orchard bees ( Osmia lignaria ) and compared microbial communities in flowers, adult bee guts, and pollen provisions before and after inoculation to determine microbial establishment, environmental filtering, and overlap across habitat types. We also inoculated provisions with microbes to test their effects on larval survival and development. Experimentally inoculated microbes were detected in all habitats, demonstrating that flowers are a source of microbial acquisition for adult and larval bees. Additionally, larval health was not impacted by microbe supplementation, indicating tolerance of bee larvae to floral microbes in Osmia .

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.002
Threshold uncertainty score0.007

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0020.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.027
GPT teacher head0.205
Teacher spread0.178 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2025
Admission routes1
Has abstractyes

Explore more

Same venuebioRxiv (Cold Spring Harbor Laboratory)→Same topicPlant and animal studies→French-language works237,207→