Serotonergic and immunomodulatory properties of the psychobiotic candidate <i>Bacteroides finegoldii</i> UO.H1052 and its extracellular vesicles
Bibliographic record
Abstract
ABSTRACT Bacteroides finegoldii UO.H1052, a human gut commensal, was evaluated for its potential psychobiotic and immunomodulatory properties. Whole-genome analysis confirmed the absence of virulence factors, plasmids, and antibiotic-resistance genes. Metabolomic profiling of cell-free supernatants (CFSs) and extracellular vesicle (EV) postbiotics revealed a high- and medium-dependent production of neuroactive metabolites, including γ-aminobutyric acid, tryptophan, tyrosine, and tyramine, as well as physiologically relevant levels of short-chain fatty acids, such as acetate, propionate, and butyrate. Functionally, CFS enhanced epithelial barrier integrity by increasing transepithelial electrical resistance and mitigating LPS-induced disruption in Caco2/HT29 monolayers without cytotoxic effects. Both CFS and EVs exhibited immunomodulatory properties, characterized by elevated Il-10 / Tnf-α ratios under basal conditions and significant suppression of Tnf-α expression in LPS-stimulated RAW 264.7 macrophages. Notably, CFS and EVs increased tryptophan hydroxylase 1 ( Tph1 ) gene expression in enterochromaffin RIN14B cells by 6.6- and 3.2-fold, respectively, suggesting enhanced serotonergic activity. These findings highlight B. finegoldii UO.H1052 as a promising next-generation psychobiotic candidate with neuroactive, barrier-protective, and immunoregulatory properties, supporting its potential for gut-brain axis modulation. IMPORTANCE Emerging evidence supports the critical role of the gut microbiota in modulating host neurophysiology and immune function via the gut-brain axis. Here, we present a comprehensive characterization of Bacteroides finegoldii UO.H1052, a human gut commensal that exhibits promising psychobiotic attributes, including the production of neuroactive compounds and extracellular vesicles (EVs) with immunoregulatory and serotonin-inducing properties. The strain exhibits a favorable safety profile, with no detected virulence factors or transmissible antibiotic resistance. Importantly, cell-free supernatants and EVs enhanced epithelial barrier integrity, modulated pro- and anti-inflammatory cytokine responses, and significantly upregulated the expression of Tph1 , a key enzyme in serotonin biosynthesis. These findings underscore the potential of B. finegoldii UO.H1052 as a next-generation psychobiotic candidate and highlight EVs as effective postbiotic mediators of host-microbe communication. This study advances the understanding of Bacteroides -derived psychobiotics and provides a foundation for their development in modulating gut-brain and immune pathways relevant to neuroinflammatory and gastrointestinal disorders.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".