<i>Mesorhizobium huakuii</i> 7653 R regulates the endophytic bacterial communities and disease resistance pathways to promote clubroot resistance in <i>Brassica napus</i>
Why this work is in the frame
A frame that forgets how it found something cannot be audited. These are the routes that admitted this work.
Bibliographic record
Abstract
Clubroot is a global soilborne disease caused by Plasmodiophora brassicae that severely affects cruciferous crops. Currently, there are no effective control measures to completely eliminate pathogens in the field. Pot experiments have shown that treatment with Mesorhizobium huakuii 7653 R can relieve clubroot symptoms. However, whether M. huakuii 7653 R can be used in the field to control clubroot disease is unclear, and the mechanism of its resistance remains largely unknown. We conducted two seasons of field trials with Brassica napus and found that M. huakuii 7653 R treatment significantly reduced clubroot disease severity, and that the agronomic traits at the seedling, flowering, and harvesting stages were significantly improved compared to the control group. The average yield per hectare, thousand grain weight, and oil content increased by 83.1%, 10.4%, and 3.7%, respectively. M. huakuii 7653 R also enhanced the resistance and yield of the cruciferous plant Brassica campestris var. purpuraria to clubroot. We characterized the composition and structure of the endophytic microbiota using bacterial 16S sequencing, and showed that treatment with M. huakuii 7653 R shaped the structure of endophytic bacterial communities. The relative abundances of Actinobacteria and Bacteroidetes, which are resistant to oxidative stress, were enriched in the co-inoculation with M. huakuii 7653 R and P. brassicae on B. napus. Transcriptome data indicated that genes related to plant immunity, nitrogen uptake and metabolism were significantly up-regulated by co-inoculation with M. huakuii 7653 R and P. brassicae. Our findings provide a theoretical basis for the widespread application of M. huakuii 7653 R in clubroot biocontrol.
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.001 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.001 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it