Anemonin is one of two anti-mitotic activities present in extracts from the Canadian Prairie Crocus, Pulsatilla nuttalliana
Bibliographic record
Abstract
We are investigating plants from the prairie ecological zone of Canada to identify natural products that inhibit mitosis in cancer cells. Extracts prepared from the Canadian prairie plant species Pulsatilla nuttalliana (Ranunculaceae) exhibited anti-mitotic activity on human cancer cell lines. P. nuttalliana -treated cells acquired a rounded morphology and were positive for phospho-histone H3, a mitotic protein. Further investigation revealed that some arrested cells displayed mitotic spindles, whereas others lacked detectable spindles. Fractionation of the extract prepared from plant stems revealed two distinct anti-mitotic activities, each of which exhibited different effects on spindle organization. Using biology-guided fractionation, we isolated one of the anti-mitotic compounds as the natural product anemonin and are the first to report its anti-mitotic activity. In addition, this is the first report of two distinct anti-mitotic activities in one botanical species and contributes to a growing body of evidence that select Canadian prairie plants have a range of anti-mitotic activities. • The first report of two distinct anti-mitotic activities from one botanical species. • The first report of the anti-mitotic activity of the natural product anemonin. • Canadian plant species hold tremendous potential as sources of natural products.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.001 | 0.000 |
| Science and technology studies | 0.001 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.002 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".