Second harmonic generation of myofibrils exhibits a polarization-resolved “gradient” effect
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Bibliographic record
Abstract
Polarization-resolved second harmonic generation microscopy (PSHG) was used to investigate the ultrastructure of individual myofibrils and collagen fibrils to visualize the ρ parameter. A gradient in the ρ values was experimentally observed orthogonal to the myofibrils, and numerical focal volume simulations were performed, indicating myosin SHG has an imaginary chiral component, similar to collagen fibrils. Both types of fibrils were re-imaged after a 180° rotation to investigate the physical mechanism behind the gradient effect. A change in the gradient direction was often observed in myofibrils, but less so in collagen, where it was more variable. PSHG is often used to discriminate muscle and collagen diseases, and chiral SHG contributions, which are typically ignored, should be considered when interpreting PSHG data.
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Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it