The nuclear localization of Ect2 is required for cytokinesis
Bibliographic record
Abstract
Abstract In cytokinesis, a contractile ring constricts the cell to form two daughters. After ingression, the ring matures into the midbody, and an intercellular bridge connects the daughter cells until it is cut during abscission. Ring assembly requires the activation of RhoA GTPase at the equatorial cortex by Ect2, a guanine nucleotide exchange factor (GEF). However, it is unclear if RhoA must be inactivated after ring closure to complete cytokinesis. Here, we show that the nuclear sequestration of Ect2 after ingression is required for cytokinesis. Mutating the nuclear localization signal (NLS) in Ect2 causes it to remain at the midbody where it generates persistent active RhoA, leading to cytokinesis failure. Re-localizing the mutant to the nucleus using SV40NLS restores cytokinesis. Further, over-expression of mutant Ect2 causes cytokinesis failure, which is rescued by reducing GEF activity. We propose that persistent RhoA causes instability in the intercellular bridge, preventing abscission. Nuclear sequestration may regulate the function of other contractile proteins with NLS sequences.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.002 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".