Regulation of Oomycete Autophagy, Lipid Droplet Accumulation and Pathogenesis by Three Rab <scp>GTPases</scp>
Bibliographic record
Abstract
Among eukaryotes, Rab GTPases are critical for intracellular membrane trafficking and possess various functions. Oomycetes, responsible for many devastating plant diseases, pose a significant threat to global agriculture. However, the functions of Rab GTPases in oomycetes are largely uncharted. In this study, we functionally characterised two Rab8 homologues, PlRab8A and PlRab8B, from a plant-pathogenic oomycete, Peronophythora litchii. These genes are crucial for mycelial growth and play significant roles in the production and morphology of sporangia, as well as the formation of zoospores. PlRab8A and PlRab8B also contribute to maintaining cell wall integrity, osmotic stress tolerance and oospore formation, which are vital for the organism's survival and virulence. Moreover, PlRab8A and PlRab8B are essential for the full virulence of P. litchii by influencing laccase activity. We also found that PlRab8B co-localised with lipid droplets (LDs) and negatively affected LD accumulation. Autophagy was enhanced and autophagy-related genes (PlATGs) were up-regulated in PlRab8B mutants. Additionally, PlRab8A was found to interact with PlRab6. Similar to PlRab8, PlRab6 is also required for growth, zoospore formation and pathogenicity of P. litchii. Taken together, this study reported for the first time the functions of three Rab proteins regulating autophagy, LD accumulation and pathogenesis in oomycetes.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".