CSDE1 promotes passenger strand cleavage of miR-486
Bibliographic record
Abstract
ABSTRACT Strand separation of the RNA duplex is a critical step in miRNA biogenesis, in which only the guide strand is retained to form the mature miRISC. The erythroid miRNA miR-486-5p uniquely requires AGO2 catalytic slicing of its passenger strand miR-486-3p, yet the factors regulating this process remain poorly understood. Here, we identify the RNA-binding protein CSDE1 as a key cofactor that promotes AGO2-dependent passenger-strand removal and miR-486-5p maturation. CSDE1 interacts with the N-terminal domain of AGO2, essential for strand release. Loss of CSDE1 increased the miR-486-3p/5p ratio, reduced cleavage efficiency, and derepressed miR-486 targets such as FOXO1 and PTEN. Restoring full-length CSDE1 expression restored AGO2-dependent passenger strand cleavage and this function requires the N-terminal cold-shock domain CSD1, which mediates CSDE1 interaction with AGO2. Together, these results demonstrate CSDE1 as a critical regulator of AGO2-mediated strand separation, potentiating miR-486-5p function in target gene silencing associated with in hematological malignancies.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.002 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".