Investigation of the effect of yeast NADH dehydrogenase (NDI1) on the radiation-induced bystander response in HCT116 cells with wild-type p53 function
Bibliographic record
Abstract
Purpose Radiation-induced bystander effects describe the biological effects in unirradiated cells induced by signals from nearby radiation-targeted cells. Although the bystander effect has been well demonstrated in cell cultures, the mechanisms underlying how bystander signaling induces mitochondrial dysfunction and metabolic alterations are not fully understood. Of interest to this study is the impairment of mitochondrial Complex I’s function in non-targeted mammalian cells.Methods NADH dehydrogenase from Saccharomyces cerevisiae (yeast) (NDI1) has been shown to restore functionality to mitochondria where complex I is inactive. In this study, NDI1 or an empty vector control was transfected into bystander human colon carcinoma cells with wild-type p53 function (HCT116 p53+/+) to investigate the effect heterologous complex has on cellular response following direct and indirect Cs-137 gamma irradiation.Results HCT116 p53+/+ were successfully transfected with either the empty vector control or NDI1 vector. NDI1 expressing cells showed no significant decrease in clonogenic survival after exposure to irradiated cell conditioned medium compared to controls.Discussion Our study suggests the alternative respiratory enzyme is able to prevent the reduction in colony formation in bystander cells. Yeast NDI1 expression may present a method to study the mechanisms by which bystander signals modulate Complex I activity. However, we cautiously note the results are preliminary in nature, and this work is an exploratory step toward mechanistic validation rather than conclusive evidence. There is a need for further validation to confirm mitochondrial localization and function of NDI1.
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.002 | 0.002 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.001 | 0.001 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".