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Record W4414830661 · doi:10.1093/jas/skaf300.512

PSXI-25 An evaluation of plasma folate and vitamin B12 concentrations of newly received feedlot heifers.

2025· article· en· W4414830661 on OpenAlexaff
M. Duplessis, Mackenzie M Smithyman, Glenn C Duff, D.H. Kleinschmit, M. E. Branine

Bibliographic record

VenueJournal of Animal Science · 2025
Typearticle
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicGenetic and phenotypic traits in livestock
Canadian institutionsAgriculture and Agri-Food Canada
Fundersnot available
KeywordsVitamin B12FeedlotBovine respiratory diseaseBlood plasmaPlasma concentrationFlorfenicolBlood sampling

Abstract

fetched live from OpenAlex

Abstract Newly received feedlot calves are at increased risk to bovine respiratory disease (BRD) due to multiple stressors including transportation and adaptation to novel environments and diet which can adversely influence metabolism and immunity. The objective was to evaluate plasma folate and vitamin B12 (B12) concentrations at feedlot arrival (d0) and 28 days later (d28) on 39 crossbred heifers (initial BW of 215 ± 30 kg) in relation to BRD morbidity. The DART (depression, appetite, respiration, and temperature) scoring system was daily assessed by three individuals to evaluate BRD morbidity status. A DART score ≥ 2 required antibiotic treatment. Throughout the 28-day period, the number of treatments was recorded and used to classify morbidity: 0 = no treatment (n=8); 1= one treatment (n=14); and 2 = two treatments or more (n=17). An indwelling temperature probe was vaginally inserted to measure vaginal temperature every 10 minutes for 28 days. Daily intake was measured by C-Lock SmartFeed system. Blood samples were collected by jugular venipuncture and BW recorded at d0 and d28. Plasma folate and B12 concentrations were analyzed by chemiluminescence. Proc MIXED of SAS with repeated measurements was used to analyze plasma folates at d0 and d28 with morbidity class and sampling time and its interaction as fixed effects. Proc CORR was used to evaluate the relationship with plasma folates and variables. Several samples were below the B12 detection limit of 150 pg/mL, therefore, proportion of samples below the detection limit was analyzed using Proc GLIMMIX with morbidity class as fixed effect, separately for d0 and d28. The interaction morbidity class x time tended to be significant for plasma folates (P=0.07). Plasma folates averaged 9.52 [standard error (SE): 1.13] ng/mL at d0 (P=0.73). At d28, it averaged 8.50, 6.41, and 5.43 (SE: 0.62) ng/mL for morbidity class 0, 1, and 2, respectively (P=0.001). Plasma folates tended to be higher for morbidity class 0 than 1 (P=0.10) and was higher for morbidity class 0 than 2 (P=0.003); with no difference between morbidity class 1 and 2 (P=0.63). Plasma folates at d28 were positively associated with average daily gain and DM intake (P=0.002). At d0, there was no difference in the proportion of samples below the detection limit for B12 between morbidity classes (P=0.70). At d28, heifers classified as morbidity class 2 had a lower proportion of samples below the detection limit for B12 [41 (SE: 12)%] than heifers in morbidity class 0 [88 (SE: 12)%] and 1 [86 (SE: 9)%, P=0.03]. It suggests that plasma B12 was higher for animals in morbidity class 2, which is in opposition with plasma folates. Additional research is required to confirm these results and evaluate potential implications on health and productivity.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Observational · Consensus signal: Observational
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.005
Threshold uncertainty score0.011

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0010.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.021
GPT teacher head0.304
Teacher spread0.282 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designObservational
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2025
Admission routes1
Has abstractyes

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