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Record W4414870593 · doi:10.1101/2025.10.03.680377

RamanBot: Versatile high throughput Raman system

2025· preprint· en· W4414870593 on OpenAlexaff
Khaled S. R. Atia, R.A. Hunter, Meshach Asare-Werehene, Benjamin K. Tsang, Hanan Anis

Bibliographic record

VenuebioRxiv (Cold Spring Harbor Laboratory) · 2025
Typepreprint
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicSpectroscopy Techniques in Biomedical and Chemical Research
Canadian institutionsOttawa HospitalUniversity of WaterlooUniversity of Ottawa
Fundersnot available
KeywordsRaman spectroscopySIGNAL (programming language)Multi-mode optical fiberThroughputSpectrometerLaserSample (material)Spectroscopy

Abstract

fetched live from OpenAlex

Abstract Raman spectroscopy is a powerful tool for qualitative and quantitative analysis in various scientific and industrial fields. However, the development of multisample automated screening remains relatively unexplored. In this paper, we develop RamanBot, a low-cost, easy-to-assemble, and automated Raman spectroscopy system designed for efficient signal collection from samples stored in different types of containers. For the first time, the proposed device introduces the Cartesian motion system, commonly used in 3D printers, to Raman spectroscopy automation. This is achieved by replacing the extrusion head of a commercially available 3D printer with a novel designed “Raman Head”. The Raman head integrates all the necessary optical components required for in-place sample excitation and signal collection. A multimode fiber is used to deliver the excitation laser to the Raman head, whereas the collected Raman signal is delivered to the spectrometer via a fiber bundle. The motion system is programmed to scan predefined sample arrangements using the standard programming language for computer numerical control (G-code). The effect of movement precision on the Raman signal is studied. The introduced device is used in the quantitative analysis of ethanol and methanol. In addition, RamanBot is used to screen six eggs in their commercial packaging with minimal human intervention. The results show that the system is highly stable and capable of delivering reliable Raman measurements, making it a promising solution for high-throughput Raman spectroscopy applications.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.001
metaresearch head score (Gemma)0.001
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Methods · Consensus signal: Methods
Teacher disagreement score0.009
Threshold uncertainty score0.029

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0010.001
Meta-epidemiology (narrow)0.0010.001
Meta-epidemiology (broad)0.0010.000
Bibliometrics0.0010.000
Science and technology studies0.0000.000
Scholarly communication0.0010.001
Open science0.0020.002
Research integrity0.0010.001
Insufficient payload (model declined to judge)0.0090.006

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.009
GPT teacher head0.260
Teacher spread0.251 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreMethods

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2025
Admission routes1
Has abstractyes

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Same venuebioRxiv (Cold Spring Harbor Laboratory)Same topicSpectroscopy Techniques in Biomedical and Chemical ResearchFrench-language works237,207