Monitoring the Uptake and Localization of Organic Compounds in Plant Tissues Using a Hydroponic <sup>14</sup>C-radiolabelling Assay and Phosphor Imaging
Bibliographic record
Abstract
C-naphthenic acids, are used to trace their absorption, translocation, localization, and metabolism in plant tissues. We have previously used this method with multiple plant species, including Elymus trachycaulus and Salix interior. These observations are corroborated here with the model plant, Arabidopsis thaliana, grown hydroponically in modified Hoagland solutions. Radiolabel uptake was monitored via liquid scintillation counting and phosphor-imaging, which allows for visualization and quantification of radiolabeled compounds within plant tissues. This method details the preparation of plant materials, the use of radiolabeled compounds, and the process of analyzing the distribution and fate of contaminants within plants. The method also includes strategies for assessing compound exudation and allows for the evaluation of both plant uptake and translocation of environmental contaminants. This approach provides insight into plant-mediated remediation processes and can be applied to the study of a wide range of environmental contaminants and plant species.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.001 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.001 | 0.001 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.001 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".