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Record W4415221468 · doi:10.1021/acs.jmedchem.5c01454

Giving an Enzyme Scissors: Serotonin Derivatives as Potent Organocatalytic Switches for DNA Repair Enzyme OGG1

2025· article· en· W4415221468 on OpenAlexfundno aff
Marek Varga, Florian Ortis, Alicia del Prado, Alice Eddershaw, Emma Rose Scaletti, Emily C. Hank, Kaixin Zhou, Natálie Rudolfová, Alessia Dodaro, Elisée Wiita, Ingrid Almlöf, Stella Karsten, Kirill Mamonov, Olov Wallner, Evert Homan, Martin Scobie, Thomas Helleday, Mario Prejanò, Pål Stenmark, Miguel de Vega, Allan J. B. Watson, Maurice Michel

Bibliographic record

VenueJournal of Medicinal Chemistry · 2025
Typearticle
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicDNA Repair Mechanisms
Canadian institutionsnot available
FundersInnovative Medicines InitiativeDr Åke Olssons Stiftelse för Haematologisk ForskningUniversità della CalabriaScience for Life LaboratoryVetenskapsrådetKarolinska InstitutetEuropean Research CouncilMinisterio de Ciencia, Innovación y UniversidadesLeverhulme TrustKungliga Tekniska HögskolanStockholms UniversitetCentro de Biología Molecular Severo OchoaCancerfondenHorizon 2020 Framework ProgrammeNovo Nordisk FondenUniversity of St AndrewsEuropean Federation of Pharmaceutical Industries and AssociationsDiamond Light SourceMcGill UniversityÅke Wiberg Stiftelse
KeywordsDNA glycosylaseDNA repairAP siteEnzymeBase excision repairDNADNA damage

Abstract

fetched live from OpenAlex

The base excision repair enzyme 8-oxoguanine DNA glycosylase 1 (OGG1) plays a central role in maintaining genome integrity and mediating cellular responses to oxidative stress. As such, it represents an attractive target for pharmaceutical modulation. Small-molecule organocatalytic switches (ORCAs) greatly enhance the rate of OGG1-catalyzed cleavage of DNA abasic sites, thereby accelerating DNA repair. Here, we present the discovery and hit-to-lead optimization of a novel class of highly potent serotonin-derived ORCAs with greatly improved pharmacokinetic properties. Biochemical assays, X-ray crystallography, and molecular dynamics simulations point toward a water-mediated mechanism of activation, distinct from previously proposed Brønsted base-assisted models. These findings establish serotonin-based ORCAs as promising chemical probes and potential leads for therapeutic modulation of OGG1 in oxidative stress-driven diseases.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.001
Threshold uncertainty score0.004

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0010.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.010
GPT teacher head0.279
Teacher spread0.269 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations3
Published2025
Admission routes1
Has abstractyes

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Same venueJournal of Medicinal ChemistrySame topicDNA Repair MechanismsFrench-language works237,207