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Record W4415268940 · doi:10.1101/2025.10.15.682631

IDBac: an open-access web platform to identify bacteria and analyze relationships in culture collections using MALDI-TOF mass spectrometry

2025· preprint· en· W4415268940 on OpenAlexaff
Nyssa K. Krull, Michael Strobel, Julia Saulog, Liana Zaroubi, Bruno S. Paulo, Mandisa Timba, Gabrielle Mingolelli, Jessia Raherisoanjato, Robert A. Shepherd, Abigail F. Scott, Claire H. Fergusson, Z. Daniel, Sriya Pokharel, Sean B. Romanowski, Antonio Bencomo Hernández, Mónica Monge-Loría, Claire E. Dylla, Manasi M. Natu, Valentina Petukhova, Neha Garg, Paul R. Jensen, Adriana Blachowicz, Chelsi D. Cassilly, Lisa Guan, Cole Stevens, Jaclyn M. Winter, Shaun M. K. McKinnie, Barbara I. Adaikpoh, Skylar Carlson, E. McCauley, William W. Metcalf, Tim S. Bugni, Michael W. Mullowney, Eric G. Pamer, Matthew T. Henke, Hazel A. Barton, David Carter, Alessandra S. Eustáquio, Roger G. Linington, Laura M. Sanchez, Mingxun Wang, Brian T. Murphy

Bibliographic record

VenuebioRxiv (Cold Spring Harbor Laboratory) · 2025
Typepreprint
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicBacterial Identification and Susceptibility Testing
Canadian institutionsSimon Fraser University
FundersNational Science Foundation Graduate Research Fellowship ProgramNational Institute of General Medical SciencesNational Institutes of Health
KeywordsIdentification (biology)SubspeciesMetadataBacteriaWeb applicationMetabolite

Abstract

fetched live from OpenAlex

Abstract The identification and analysis of bacteria is central to the microbiological sciences. While gene sequencing methods have been the standard to achieve this, use of MALDI-TOF mass spectrometry (MS), particularly in clinical microbiology, provides high-throughput identification to the subspecies level. However, biotyping has yet to be adopted outside of clinical settings due to the lack of a centralized public database of MS protein signatures that would facilitate isolate identification via spectral comparison. Further, current platforms lack meaningful ways to compare multiple properties from large numbers of bacterial isolates. Herein we present the IDBac web platform, a crowd-sourced central knowledgebase of protein MS signatures of >1400 strains spanning 6 bacterial phyla. Accompanying the knowledgebase is analysis infrastructure to identify unknown isolates, probe relationships within culture collections using metadata integration, and visualize specialized metabolite differences within groups of closely related bacteria. To highlight this utility and encourage wide community contribution, examples of each are presented.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.001
metaresearch head score (Gemma)0.001
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesMeta-epidemiology (narrow), Scholarly communication
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: none
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.639
Threshold uncertainty score1.000

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0010.001
Meta-epidemiology (narrow)0.0000.001
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0010.001
Science and technology studies0.0000.000
Scholarly communication0.0020.000
Open science0.0010.002
Research integrity0.0010.001
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.055
GPT teacher head0.333
Teacher spread0.278 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one teacher head, not a consensus.

Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations1
Published2025
Admission routes1
Has abstractyes

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