Co-expression of Bacterial Hemoglobin Improves Recombinant Expression of Carbonic Anhydrase in <i>Escherichia coli</i>
Bibliographic record
Abstract
Carbonic anhydrase (CA) is a zinc-containing enzyme that catalyzes the CO 2 hydration reaction.CA has gained much attention as a green and sustainable biocatalyst to accelerate CO 2 capture and utilization.For industrial applications, it is desirable to reduce the enzyme production cost by improving the recombinant production of CA.Vitreoscilla hemoglobin (VHb) is a bacterial hemoglobin whose heterologous expression in Escherichia coli can improve cellular respiration and thereby recombinant protein production.In this study, Neisseria gonorrhoeae CA (NgCA) as a model CA was co-expressed with VHb in E. coli to improve the production of NgCA.When the VHb co-expression was controlled by the hypoxia-inducible Nar promoter, no visible VHb expression nor improvement in NgCA production was observed.In contrast, the bicistronic expression of VHb along with NgCA by the isopropyl-β-D-thiogalactopyranoside-inducible Trc promoter improved the expression level of NgCA by 15%-18%.The activity of purified NgCA was not affected by the VHb co-expression.Thus, the VHb co-expression should have general use as an effective strategy to increase the bacterial production of industrially relevant CA enzymes.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".