Co-expression of Bacterial Hemoglobin Improves Recombinant Expression of Carbonic Anhydrase in <i>Escherichia coli</i>
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Bibliographic record
Abstract
Carbonic anhydrase (CA) is a zinc-containing enzyme that catalyzes the CO 2 hydration reaction.CA has gained much attention as a green and sustainable biocatalyst to accelerate CO 2 capture and utilization.For industrial applications, it is desirable to reduce the enzyme production cost by improving the recombinant production of CA.Vitreoscilla hemoglobin (VHb) is a bacterial hemoglobin whose heterologous expression in Escherichia coli can improve cellular respiration and thereby recombinant protein production.In this study, Neisseria gonorrhoeae CA (NgCA) as a model CA was co-expressed with VHb in E. coli to improve the production of NgCA.When the VHb co-expression was controlled by the hypoxia-inducible Nar promoter, no visible VHb expression nor improvement in NgCA production was observed.In contrast, the bicistronic expression of VHb along with NgCA by the isopropyl--D-thiogalactopyranoside-inducible Trc promoter improved the expression level of NgCA by 15%-18%.The activity of purified NgCA was not affected by the VHb co-expression.Thus, the VHb co-expression should have general use as an effective strategy to increase the bacterial production of industrially relevant CA enzymes.
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Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it