A Cysteine‐Rich Secretory Protein Regulates Sensitivity of Stresses, Contributing to the Virulence of <i>Fusarium oxysporum</i> f. sp. <i>cubense</i> Race 4
Why this work is in the frame
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Bibliographic record
Abstract
ABSTRACT Fusarium oxysporum f. sp. cubense (Foc), the causative agent of banana wilt, poses a significant global threat to banana cultivation. This study identified a novel virulence effector in Foc tropical race 4 (FocTR4), FoCRISPs1, which contributed to the pathogen's ability to colonise plant tissues by navigating plant immune responses. FoCRISPs1 is characterised as a cysteine‐rich hypothetical protein comprised of 716 amino acids, and it features a functional signal peptide without recognisable motifs or domains. Disruption of FoCRISPs1 in FocTR4 notably diminished the pathogen's resilience to oxidative stress and cell wall‐targeting agents, leading to attenuated virulence toward banana plants. Subcellular localisation in Nicotiana benthamiana cells revealed FoCRISPs1 predominantly in the cytoplasm, where it effectively suppressed BAX‐induced programmed cell death. The FoCRISPs1 deletion mutants exhibited significant reductions in pathogenicity, highlighting FoCRISPs1's integral role in enhancing the pathogenicity of FocTR4. By bolstering resistance to environmental stressors and dampening host defence mechanisms, FoCRISPs1 was found to be a critical factor in the disease dynamics of banana wilt. These findings not only shed light on the molecular underpinnings of FocTR4's virulence, but they also offer potential avenues for developing targeted disease management strategies.
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Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.001 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it