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Record W4415533258 · doi:10.1101/2025.10.23.684134

HDCluster: High-Degree Graph Clustering for Robust Analysis of Single Molecule Localization Microscopy

2025· preprint· en· W4415533258 on OpenAlex
Ismail M. Khater, Ivan R. Nabi, Ghassan Hamarneh

Why this work is in the frame

A frame that forgets how it found something cannot be audited. These are the routes that admitted this work.

affAt least one author lists a Canadian institution in the pinned OpenAlex snapshot.
fundA Canadian funder is recorded on the work.

Bibliographic record

VenuebioRxiv (Cold Spring Harbor Laboratory) · 2025
Typepreprint
Languageen
FieldMaterials Science
TopicMachine Learning in Materials Science
Canadian institutionsMcGill UniversityUniversity of British ColumbiaUniversité de MontréalSimon Fraser University
FundersNatural Sciences and Engineering Research Council of CanadaCanadian Institutes of Health ResearchNational Institutes of HealthBirzeit University
KeywordsCluster analysisCorrelation clusteringCURE data clustering algorithmSingle-linkage clusteringContext (archaeology)Fuzzy clusteringPattern recognition (psychology)Cluster (spacecraft)

Abstract

fetched live from OpenAlex

Clustering is a fundamental task in data analysis: grouping similar objects together into distinguishable subsets. Here, we introduce HDCluster, a novel high-degree graph-based clustering algorithm designed to effectively and rapidly handle various real-world clustering applications, particularly in the context of super-resolution single molecule localization microscopy (SMLM). HDCluster efficiently handles datasets with large and variable numbers of clusters, without requiring prior knowledge of the cluster count, relying on only one parameter. The high speed and efficiency of HDCluster allow it to handle large SMLM datasets with millions of localizations. A comprehensive quantitative comparison against state-of-the-art clustering methods using simulated, public, and real-world datasets demonstrates that HDCluster outperforms other clustering algorithms in terms of time efficiency and clustering performance measures, such as ARI and AMI. HDCluster is particularly robust to noise, making it a promising and effective tool for various clustering tasks in big-data settings, such as SMLM.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.002
metaresearch head score (Gemma)0.001
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesMeta-epidemiology (narrow)
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: none
Teacher disagreement score0.416
Threshold uncertainty score1.000

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0020.001
Meta-epidemiology (narrow)0.0010.001
Meta-epidemiology (broad)0.0010.000
Bibliometrics0.0010.002
Science and technology studies0.0000.000
Scholarly communication0.0010.000
Open science0.0020.001
Research integrity0.0010.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.019
GPT teacher head0.249
Teacher spread0.230 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it