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Record W4415641952 · doi:10.1101/2025.10.20.25337094

A multicentre study to predict COVID-19 outbreaks in long-term care homes using wastewater surveillance and environmental surface sampling for SARS-CoV-2

2025· preprint· W4415641952 on OpenAlexafffundabout
Jason Moggridge, Derek R. MacFadden, Alex Wong, Rees Kassen, Caroline Nott, Gustavo Ybazeta, David S. Guttman, Lucas Castellani, Michael Fralick

Bibliographic record

VenuemedRxiv · 2025
Typepreprint
Language
FieldMedicine
TopicSARS-CoV-2 detection and testing
Canadian institutionsNOSM UniversitySinai Health SystemCarleton UniversityUniversity of TorontoHealth Sciences NorthMcGill UniversityOttawa Hospital
FundersUniversity of Toronto
KeywordsOutbreakLogistic regressionWastewaterSampling (signal processing)Sewage treatmentCoronavirus disease 2019 (COVID-19)

Abstract

fetched live from OpenAlex

ABSTRACT Background Floor swabs can be an effective environmental sampling method for proactive SARS-CoV-2 surveillance in congregate settings like long-term care homes (LTCHs). Concurrent assessment of additional variables such as wastewater surveillance data and weather data have the potential to improve the predictive performance of this approach. Methods We analyzed existing data from 5,095 floor swabs collected between August 2021 and January 2023 from 10 LTCHs across three cities in Ontario, Canada: Ottawa, Toronto, and Sault Ste. Marie. Floors were swabbed weekly at each LTCH. Swabs were analyzed using RT-qPCR. Wastewater data was obtained from the Ontario Wastewater Surveillance Consortium’s repository; we included one treatment plant for each city. Weather data was sourced from Environment Canada, with one station selected from each city. Logistic regression, LASSO-penalized logistic regression, Random Forest, and XGBoost were used for COVID-19 outbreak predictions using different subsets of predictors with leave-one-LTCH-out cross-validation. SHAP values were computed for model explainability. Our outcome of interest was a COVID-19 outbreak within an LTCH. Results Over the study period, 25 COVID-19 outbreaks occurred in the participating LTCHs, with a median duration of 30 days and a median of 39 cases per outbreak (range 2 to 196). LASSO generally out-performed logistic regression, Random Forest, and XGBoost. The two variables with the highest SHAP values were log transformed 7-day mean wastewater and log viral copies from floor swabs. Conclusions Incorporating wastewater data and weather data enhanced the ability of floor swab results to predict an outbreak of COVID-19 in an LTCH. Future studies are needed to evaluate how well the model performs when implemented into practice.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.003
metaresearch head score (Gemma)0.004
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Observational · Consensus signal: Observational
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.145
Threshold uncertainty score0.288

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0030.004
Meta-epidemiology (narrow)0.0010.001
Meta-epidemiology (broad)0.0010.001
Bibliometrics0.0010.001
Science and technology studies0.0010.001
Scholarly communication0.0010.001
Open science0.0010.001
Research integrity0.0010.001
Insufficient payload (model declined to judge)0.0010.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.064
GPT teacher head0.358
Teacher spread0.294 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designObservational
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2025
Admission routes3
Has abstractyes

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