Protocol for dissection and organotypic explant culture of the neonatal and adult stria vascularis
Bibliographic record
Abstract
Comprehensive analysis of the stria vascularis (SV), an essential structure for hearing function, would greatly benefit from an in vitro experimental platform. Here, we present a protocol for dissection and isolation of the neonatal and adult mouse SV (focusing on post-natal day [P]0–35) from base to apex. We then detail procedures for explanting the SV on Matrigel-coated well dishes, followed by washing and fixing procedures. For complete details on the use and execution of the protocol, please refer to Thulasiram et al. 1 • Step-by-step dissection of inner ears from neonatal and mature mice • Isolation and in vitro explant culture of the stria vascularis from base to apex • Basic and preclinical research platform for stria vascularis-related hearing loss Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. Comprehensive analysis of the stria vascularis (SV), an essential structure for hearing function, would greatly benefit from an in vitro experimental platform. Here, we present a protocol for dissection and isolation of the neonatal and adult mouse SV (focusing on post-natal day [P]0–35) from base to apex. We then detail procedures for explanting the SV on Matrigel-coated well dishes, followed by washing and fixing procedures.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.001 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".