Widespread emergence of <i>Staphylococcus aureus</i> with variant FarR regulators and enhanced resistance to antimicrobial fatty acids within clonal complex CC5, CC8, and CC97 strains from human and bovine hosts
Bibliographic record
Abstract
ABSTRACT The FarER efflux pump and regulator confer inducible resistance of Staphylococcus aureus to linoleic acid, an unsaturated antimicrobial fatty acid that comprises a chemical component of innate immunity at sites of colonization and infection. We previously discovered farR through in vitro selection for increased resistance to linoleic acid, leading to recovery of FarR H121Y that promoted enhanced expression of farE . Here, we report on the widespread emergence of increased resistance to linoleic acid across the phylogenetic spectrum of S. aureus through mechanisms that are both dependent and independent of farER . In this latter group, sequence type ST228 healthcare-associated methicillin-resistant S. aureus has FarR A14P that is defective in DNA binding and cannot induce farE expression, and yet these strains exhibit enhanced resistance to linoleic acid. In the former group, we note the polyphyletic emergence of FarR G166D in ST5 community-acquired MRSA (CA-MRSA), adding to previously noted variants FarR C116Y and FarR E160G in ST5 MRSA. We also observe FarR H121Y and FarR G166D in ST97 methicillin-susceptible S. aureus from bovine hosts, together with polyphyletic emergence of FarR C116Y . Last, we note the emergence of FarR H121Y in the USA300 lineage of ST8 CA-MRSA from the UK, in association with an SCC mec VI element that encodes resistance to fusidic acid, a topical antimicrobial used to treat superficial skin infections. Our data support the widespread emergence and dissemination of S. aureus strains from both human and bovine hosts, with enhanced resistance to unsaturated free fatty acids that comprise an important chemical component of innate immunity. IMPORTANCE Staphylococcus aureus is a priority pathogen for the World Health Organization due to its association with human morbidity, mortality, and acquisition of multiple drug resistance. In addition to acquiring specific antimicrobial resistance genes, it is also widely documented that S. aureus can evolve through accumulation of genetic polymorphisms that confer reduced susceptibility to some antibiotics such as daptomycin and vancomycin. In this study, we report on the widespread emergence of S. aureus strains with sufficiency for increased resistance to antimicrobial fatty acids through polymorphisms in the regulator of an efflux pump. This is an indication that, in addition to evolution in response to antibiotics that are used to treat infections, S. aureus also exhibits the capacity to evolve in response to antimicrobial unsaturated fatty acids that are important chemical components of innate immunity. These findings underscore the exceptional capacity of S. aureus to evolve in response to antimicrobial threats.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.001 | 0.001 |
| Meta-epidemiology (broad) | 0.001 | 0.000 |
| Bibliometrics | 0.000 | 0.001 |
| Science and technology studies | 0.000 | 0.002 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".