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Record W4416094434 · doi:10.1242/jeb.250502

Impact of acute and chronic hypoxia on the heme oxygenase/carbon monoxide pathway in naked mole-rats ( <i>Heterocephalus glaber</i> )

2025· article· en· W4416094434 on OpenAlexafffund
Kristi M. Kezar, Liam Eaton, Karen L. Kadamani, Mohammad Ojaghi, Leo E. Otterbein, Matthew E. Pamenter, Michael S. Tift

Bibliographic record

VenueJournal of Experimental Biology · 2025
Typearticle
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicHeme Oxygenase-1 and Carbon Monoxide
Canadian institutionsUniversity of Ottawa
FundersNatural Sciences and Engineering Research Council of CanadaUniversity of North Carolina WilmingtonCanada Research ChairsCanada Excellence Research Chairs, Government of CanadaDivision of Integrative Organismal SystemsNational Football League Players AssociationUniversity of OttawaNational Science Foundation
KeywordsHeme oxygenaseHypoxia (environmental)HemeKidneyHemoglobinSpleenCarbon monoxideLung

Abstract

fetched live from OpenAlex

Heme oxygenase (HO) enzymes are responsible for the degradation of free heme and producing endogenous carbon monoxide (CO). Research has suggested that the HO-CO pathway imparts protective effects to hypoxic tissues. The objective of this study was to investigate the effects of acute (4 h or 24 h at 7% O2) and chronic (7 days at 11% O2) hypoxia on the HO-CO pathway in the hypoxia-tolerant naked mole-rat. Specifically, we measured CO concentrations in nine organs and blood, as well as HO activity in all organs of animals exposed to normoxia (21% O2), acute hypoxia or chronic hypoxia. Hypoxia did not impact CO concentration or HO activity in most tissues, with the exception of the brain (decreased [CO] after 24 h and 7 days), heart (increased HO activity after 4 h), and intestine (increased [CO] after 24 h and 7 days but decreased HO activity after 24 h). Relative to normoxic controls, hemoglobin concentrations increased 10-12% in animals exposed to acute hypoxia but were unchanged following chronic hypoxia. In naked mole-rats exposed to normoxia or hypoxia, CO concentrations were higher in blood, lung and spleen, and HO activity was higher in the kidney and lung when compared with tissues from mice exposed to normoxia. However, splenic HO activity was higher in mice exposed to normoxia when compared with spleen from naked mole-rats in all treatment conditions. Unlike non-hypoxia tolerant species, chronic hypoxia did not suppress the HO-CO pathway in naked mole-rats, highlighting the importance of this pathway in hypoxia physiology.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.002
Threshold uncertainty score0.004

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0010.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.001
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.001
Insufficient payload (model declined to judge)0.0010.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.009
GPT teacher head0.282
Teacher spread0.273 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations1
Published2025
Admission routes2
Has abstractyes

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