High-speed neural imaging with multiplexed miniaturized two-photon microscopy
Why this work is in the frame
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Bibliographic record
Abstract
Head-mounted miniaturized two-photon microscopes enable cellular-resolution recording of neural activity deep in the mouse brain during unrestrained behavior. Two-photon microscopy, however, is traditionally limited in frame rate by the necessity of scanning the excitation beam over a large field-of-view (FOV). Here, we present two types of multiplexed miniaturized two-photon microscopes (M-MINI2Ps) that preserve spatial resolution while increasing frame rate by simultaneously imaging two FOVs and demixing them temporally or computationally. We demonstrate large-scale (500 × 500 μm 2 FOV) multiplane calcium imaging in visual and prefrontal cortices of freely moving mice during spontaneous exploration, social behavior, and auditory stimulus. The increased speed of M-MINI2Ps also enables two-photon voltage imaging at 400 Hz over a 380 × 150 μm 2 FOV in freely moving mice. With compact footprints and compatibility with the open-source MINI2P, M-MINI2Ps enable high-speed recording of rapid neural dynamics and large-volume population activity in freely moving mice, providing a powerful tool for systems neuroscience.
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Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.001 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.001 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it