Rectal Carriage of Sequence Type 307 <i>Klebsiella pneumoniae</i> High‐Risk Clone Harboring Multiple Carbapenemase Genes in Community Hospitals Gauteng, South Africa
Why this work is in the frame
A frame that forgets how it found something cannot be audited. These are the routes that admitted this work.
Bibliographic record
Abstract
ABSTRACT Asymptomatic rectal carriers are recognized as reservoirs of carbapenem‐resistant Klebsiella pneumoniae (CRKp), which can spread epidemic high‐risk clones [e.g., sequence types (ST)‐307] and plasmids [incompatibility group (Inc)‐X3] in hospitals, with possible transmission into the community. This study investigated the epidemiology and characteristics of CRKp high‐risk clones ST307 among rectal carriage isolates from community hospitals. A carbapenemase positivity rate of 24% was observed for all rectal screening performed during hospital admission (February to August 2021) in Gauteng, South Africa; 252 CRKp isolates were characterized. Antimicrobial susceptibility was performed using the VITEK 2 automated system, and polymerase chain reaction assays were used to detect K. pneumoniae ST307, carbapenemase genes, and associated mobile genetic elements (MGEs e.g., IncX3, IS3000). Of the 252 isolates, 25% (64/252) were ST307 positive and 75% (188/252) were non‐ST307. Among the 64 ST307, 45% (29/64) harbored bla OXA‐181 on IncX3 plasmids. Occurrence of bla OXA‐181 among ST307 (69%; 44/64) when compared to non‐ST307 (48%; 91/188) was statistically significant ( p ‐value = 0.002). Fourteen isolates, including two ST307, harbored double carbapenemase genes. Carbapenemase gene combinations include six bla NDM +bla OXA‐48‐like , four bla NDM +bla OXA‐181 , three bla KPC +bla OXA‐181 , and one bla OXA‐181 + bla VIM . One ST307 isolate harbored three carbapenemase genes ( bla NDM +bla OXA‐48 +bla OXA‐181 ). Level of antimicrobial resistance was significantly ( p ‐value < 0.001) associated with the occurrence of ST307, comprising 73% (47/64) extensively drug resistant. This study highlights the need for rectal screening of XDR clones and plasmids using simple and cost‐effective genomic methodologies suitable for low‐ and middle‐income countries for local risk management and control of infectious diseases in hospitals.
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Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.001 | 0.001 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it