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Record W4416330263 · doi:10.1101/2025.11.18.689010

Soil trace gas oxidizers divergently respond to short- and long-term warming

2025· preprint· W4416330263 on OpenAlexaff
Thanh Nguyen-Dinh, Vojtěch Tláskal, Pok Man Leung, Magdalena Wutkowska, Justus Amuche Nweze, Katri Yla-Soininmaki, Johanna Kerttula, Mathilde Borg Dahl, Andrea Söllinger, Tilman Schmider, Christina Biasi, Andreas Richter, Tim Urich, Alexander Tøsdal Tveit, Chris Greening, Bjarni D. Sigurðsson, Anne Daebeler

Bibliographic record

VenuebioRxiv (Cold Spring Harbor Laboratory) · 2025
Typepreprint
Language
FieldAgricultural and Biological Sciences
TopicSoil Carbon and Nitrogen Dynamics
Canadian institutionsCentre Casa
FundersAustralian Research CouncilAcademy of FinlandNorges ForskningsrådAustrian Science FundGrantová Agentura České RepublikyDeutsche Forschungsgemeinschaft
KeywordsTrace gasBiogeochemical cycleSink (geography)Soil waterGrasslandEcosystemGlobal warmingNitrogen cycle

Abstract

fetched live from OpenAlex

Abstract The upland soil microbiome is dominated by aerobic bacteria that oxidize atmospheric trace gases, including CO, H 2 , and CH 4 . As a result, soils are the largest biological sink for these climate-active gases. Whether global warming will enhance or suppress these processes remains unclear. Here, we studied the warming responses of soil trace gas oxidizers by profiling natural geothermal gradients in a subarctic grassland with over 60 years of field warming at +6°C. We integrate field flux measurements, ex situ biogeochemical assays, metagenomics, and metatranscriptomics to determine ecosystem and cellular-level responses. Our results show that the oxidation of atmospheric CO and H 2 , but not CH 4 , increased with long-term warming due to higher cell numbers. However, at the cellular level, trace gas oxidizers, especially methanotrophs, tended to reduce gas consumption and transcription of gas-metabolizing enzymes in response to long-term warming. Our findings suggest that soils may remain a robust sink for trace gases despite lower per-cell activity. This work establishes a framework for interpreting the relationships between temperature and microbial trace gas oxidation on timescales relevant to Earth’s climate system.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Observational · Consensus signal: none
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.003
Threshold uncertainty score0.006

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0010.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0010.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.018
GPT teacher head0.230
Teacher spread0.212 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designObservational
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2025
Admission routes1
Has abstractyes

Explore more

Same venuebioRxiv (Cold Spring Harbor Laboratory)→Same topicSoil Carbon and Nitrogen Dynamics→French-language works237,207→