Genome integrity relies on rapid recycling of DNA Pol III in bacteria
Bibliographic record
Abstract
DNA replication requires precise coordination between DNA unwinding and DNA synthesis. In all domains of life, protein-protein interactions at the replisome maintain proximity between the enzymes that catalyze these two activities. Surprisingly, in bacteria, the replicative DNA polymerase (Pol III), responsible for DNA synthesis, is exchanged every few seconds. How processive synthesis is maintained under these conditions has remained unclear. Here, we use single-molecule microscopy in live cells to show that Pol III rapidly rebinds the replisome within seconds of dissociation. This fast recruitment is driven by an interaction with single-stranded DNA-binding protein (SSB), which enhances Pol III target search efficiency by ~20-fold. Disrupting this mechanism alters replisome stoichiometry, causes single-stranded DNA (ssDNA) accumulation, depletes free SSB, and leads to helicase disassembly and DNA breakage, culminating in genome instability. Our findings reveal a mechanism by which fast polymerase recycling sustains continuous DNA replication and genome integrity.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.001 | 0.000 |
| Open science | 0.000 | 0.001 |
| Research integrity | 0.001 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".