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Record W4416470791 · doi:10.1038/s41467-025-66070-7

Geographics and bacterial networks differently shape the acquired and latent global sewage resistomes

2025· article· en· W4416470791 on OpenAlexaff
Hannah-Marie Martiny, Patrick Munk, Alessandro Fuschi, Ágnes Becsei, Nikiforos Pyrounakis, Christian Brinch, D. G. Joakim Larsson, Marion Koopmans, Nawel Zaatout, Catherine A. Rees, Guangming Jiang, Jiahua Shi, Bernhard Benka, Franz Allerberger, Sandra Koeberl-Jelovcan, Khalil Hasan Abdulla, Ali Bin Thani, Anowara Begum, Carlon Worrell, Freddy Soria, Nataša Mazalica, Teddie O. Rahube, Andreza Francisco Martins, Carlos Alberto Tagliati, Larissa Camila Ribeiro de Souza, Ariane Nzouankeu, Muhammad Attiq Rehman, Jeff Gauthier, Roger C. Lévesque, Sean D. Workman, Christopher K. Yost, Aiko Adell Nakashima, Andrés Opazo-Capurro, G. Gonzales, Yongjie Yu, Pilar Donado-Godoy, Tadjidine Youssouf, Pablo-César Rivera-Navarro, Matijana Jergović, Jasna Hrenović, Renáta Karpíšková, Julien Kalpy Coulibaly, William Calero‐Cáceres, M. Abouelnaga, Anna-Maria Hokajärvi, Annamari Heikinheimo, Soizick Le Guyader, Andreas Nitsche, Annika Brinkmann, Sara Schubert, Thomas U. Berendonk, Uli Klümper, Ernest Bonah, Solomon Asante-Sefa, Andrew Camilleri, Courage Kosi Setsoafia Saba, Victoria Bernice Sedor, Kassiani Μellou, Theologia Sideroglou, Charalampos Kotzamanidis, Jens-Peter B. Henriksen, Mie Møller, Thorunn R. Thorsteinsdottir, A. A. Mohamed Hatha, Burhan Shamurad, Hiwa Ali Faraj, Dearbháile Morris, Louise O’Connor, Jacob Moran‐Gilad, Roberta Orletti, Giuseppina La Rosa, M. Iaconelli, Antonio Battisti, Lucia Decastelli, Maira Napoleoni, Alessandra De Cesare, Gianluca Corno, Jeļena Avsejenko, Ghassan M. Matar, Christian Penny, Luc Hervé Samison, Daniel L. Banda, Heera Rajandas, Sivachandran Parimannan, Malcolm Vella Haber, Sunita J. Santchurn, Julián Carrillo‐Reyes, René Arredondo‐Hernández, Aleksandar Vujacic, Dijana Djurović, Pushkar Pal, Carlos Campos, Isabelle Pattis, Stephen T. Chambers, Gert‐Jan Jeunen, Charles John Elikwu, Olayinka Osuolale, Mwapu Dika Ndahi, Oluwadamilola Abiodun-Adewusi, Amen Ekhosuehi, Ayorinde O. Afolayan, Kayode Fashae, Mabel Kamweli Aworh, Akeem Olayiwola Ahmed, Ibrahim Adisa Raufu, Ismail Ayoade Odetokun, Samaila Musa Chiroma, Claire L. Mitchell, Rune Holmstad, Natalie Weiler, Dariusz Wasyl, Magdalena Zając, Eugénia Cardoso, Célia M. Manaia, Maria Jorge Campos, Hor‐Gil Hur, Min Song, Sukhwan Yoon, Olga Burduniuc, Pei‐Ying Hong, Vladimir Radosavljević, Angel Anisa Cokro, Dagmar Gavačová, Marija Trkov, Rukayya Hussain Abubakar, Karen H. Keddy, Isabel Martínez‐Alcalá, Marta Cerdà‐Cuéllar, A Cañas, Fernando Gónzález‐Candelas, Yahia Ali Sabiel, Tanja Van der Heijden, Yu-Ping Hong, J. J. Medardus, Rajeev Nagassar, Cemil Kürekçi, Patrick J. McNamara, Erica R. Fuhrmeister, John Scott Meschke, Nicola K. Beck, Ayella Maile-Moskowitz, Jens Thomsen, Ouidiane Obaidi, Bruce Paterson, Anne Frances Clare Leonard, Lihong Zhang, Kevin Chau, Daniel Remondini, István Csabai, Frank M. Aarestrup

Bibliographic record

VenueNature Communications · 2025
Typearticle
Languageen
FieldEnvironmental Science
TopicPharmaceutical and Antibiotic Environmental Impacts
Canadian institutionsUniversity of ReginaUniversité LavalResearch and Productivity Council
FundersHorizon 2020 Framework ProgrammeNovo Nordisk FondenNovo NordiskEuropean Commission
KeywordsResistomeMetagenomicsAntibiotic resistanceSewageTaxonNicheResistance (ecology)Ecological niche

Abstract

fetched live from OpenAlex

Antimicrobial resistance genes (ARGs) have rapidly emerged and spread globally, but the pathways driving their spread remain poorly understood. We analyzed 1240 sewage samples from 351 cities across 111 countries, comparing ARGs known to be mobilized with those identified through functional metagenomics (FG). FG ARGs showed stronger associations with bacterial taxa than the acquired ARGs. Network analyses further confirmed this and showed potential for source attribution of both known and novel ARGs. The FG resistome was more evenly dispersed globally, whereas the acquired resistome followed distinct geographical patterns. City-wise distance-decay analyses revealed that the FG ARGs showed significant decay within countries but not across regions or globally. In contrast, acquired ARGs showed decay at both national and regional scales. At the variant level, both ARG groups had significant national and regional distance-decay effects, but only FG ARGs at a global scale. Additionally, we observed stronger distance effects in Sub-Saharan Africa and East Asia compared to North America. Our findings suggest that differential selection and niche competition, rather than dispersal, shape the global resistome patterns. A limited number of bacterial taxa may act as reservoirs of latent FG ARGs, highlighting the need of targeted surveillance to mitigate future resistance threats.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.001
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Observational · Consensus signal: Observational
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.003
Threshold uncertainty score0.006

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.001
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0010.001
Science and technology studies0.0000.001
Scholarly communication0.0010.001
Open science0.0000.001
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0020.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.016
GPT teacher head0.294
Teacher spread0.278 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designObservational
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations8
Published2025
Admission routes1
Has abstractyes

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