Isolation of marine bacteria through a “bait” approach
Bibliographic record
Abstract
There is a great divide between the microbes active in natural environments and the organisms that may be grown in a laboratory setting. In this work we set out to cultivate representatives of the marine myxobacterial clade, a highly diverse, largely uncultured group of Gram-negative bacteria believed to have extensive biosynthetic potential. Sediment samples were collected from the St. Lawrence Estuary and Gulf and the presence of active marine myxobacteria was established through qPCR analysis of 16S rRNA gene and transcript abundances. In the expectation that the marine myxobacteria would exhibit predatory behaviour like their terrestrial counterparts, the sediment samples were then streaked on agar plates that contained common marine bacteria as the sole carbon source. Unexpectedly, in place of myxobacteria we isolated Pseudomonas, Bacillus, and Stenotropomonas spp., among others, revealing a generalized ability for these strains to break down living organic matter and suggesting that “bait” bacteria may be an effective approach for the cultivation of novel marine saprophytes.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.001 |
| Meta-epidemiology (narrow) | 0.001 | 0.000 |
| Meta-epidemiology (broad) | 0.001 | 0.000 |
| Bibliometrics | 0.001 | 0.001 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.001 | 0.000 |
| Open science | 0.000 | 0.001 |
| Research integrity | 0.001 | 0.001 |
| Insufficient payload (model declined to judge) | 0.001 | 0.003 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".