Comparative Proteomic Analysis of Self‐Compatible <i>Turnera</i> Mutants Suggests <i>Glutathione S‐Transferase 8</i> Is Involved in Overcoming S‐Morph Female Self‐Incompatibility Response
Bibliographic record
Abstract
ABSTRACT Distyly is a reproductive system, characterized by the presence of two floral morphs, which promotes outcrossing via physical and biochemical means. In distylous Turnera , the mating type of the S‐morph is determined by two genes: YUC6 (male) and BAHD (female). Despite the importance of these S ‐genes, it is likely that additional genes are involved in the distylous syndrome. Here, we use comparative mass spectrometry analysis to identify differentially expressed proteins in a series of self‐compatible mutants and wildtype distylous members of Turnera . Our analysis identified a member of the Glutathione S‐transferase family that overwhelmingly correlated with L‐morph male mating type. Exploration of the large datasets and previously published work led to the proposal that differential ROS levels in the pistil may contribute towards the self‐incompatibility response. To support this hypothesis, we generated a co‐expression network for whole flower buds from self‐compatible and WT Turnera joelii . This network led to the identification of a series of ROS and auxin‐related genes that correlated with self‐compatibility. We update previously proposed SI response models to reflect how ROS, jasmonic acid, and brassinosteroid signaling likely establish the S‐morph female self‐incompatibility response. Overall, this work has identified genes potentially related to self‐compatibility and has provided a foundation for future empirical work investigating the basis of the SI response in Turnera .
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".