Class A and B GPCRs trigger rapid Gαs translocation to late and slow recycling endosomes
Bibliographic record
Abstract
Abstract Gα s is classically known for mediating G protein-coupled receptor (GPCR) signaling at the plasma membrane (PM), but it is now established that Gα s also supports a second wave of signaling from internalized GPCRs within early endosomes. However, the mechanisms underlying Gα s trafficking remain unclear. Here, using live-cell confocal microscopy and bioluminescence resonance energy transfer (BRET) assays, we investigated Gα s -GFP dynamics following activation of class A (β 2 AR) and class B (V 2 R) receptors, which exhibit different level of endosomal signaling. Our findings demonstrate that Gα s rapidly ( < 2 min) translocates to late (Rab7) and slow recycling (Rab11) endosomes, bypassing the classical endocytic route and displaying only transient colocalization with receptors. This trafficking depends on Gα s activation at the PM, its release from the membrane, and an intact palmitoylation site, but occurs independently of receptor internalization. This work shed light on non-canonical route for Gα s endosomal trafficking, with important implications for endosomal GPCR signaling.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.002 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".