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Record W4417007256 · doi:10.1139/cjas-2025-0074

<i>Et tu, brute force?</i> An approach to refine blood sample collection protocols

2025· article· en· W4417007256 on OpenAlexaffvenue
Dave J Seymour, C A Bertens, G.B. Penner

Bibliographic record

VenueCanadian Journal of Animal Science · 2025
Typearticle
Languageen
FieldVeterinary
TopicAnimal testing and alternatives
Canadian institutionsUniversity of SaskatchewanUniversity of Guelph
Fundersnot available
KeywordsProtocol (science)Sampling (signal processing)Sample (material)Blood samplingBioequivalenceSample size determination

Abstract

fetched live from OpenAlex

Reducing the number of samples collected on individual animals during an experiment has various advantages; however, it is critical that any refinements to a sampling protocol retain a similar degree of accuracy. The objective of this study was to develop a refined blood sampling protocol capable of reproducing the original findings of a pilot study using only a subset of the collected samples. Data from a previous study were available consisting of plasma concentrations of Cr and Co collected at 15 timepoints over a 64 h period that were used to calculate area under the curve (AUC). Prior to subsequent analysis, it was determined that an adequate reduced sampling protocol must estimate the original AUC within 10%. A SAS program was prepared that generated all possible combinations of sample timepoints. Optimal sets of timepoints were identified by minimal root mean square prediction error. Output from this procedure can be evaluated for bioequivalence using the two one-sided tests approach. This method highlights the ability to further refine biological sampling protocols, which can be readily applied to other experimental settings.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.001
metaresearch head score (Gemma)0.001
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: none
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.894
Threshold uncertainty score0.999

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0010.001
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.001
Science and technology studies0.0010.000
Scholarly communication0.0000.001
Open science0.0010.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.152
GPT teacher head0.403
Teacher spread0.251 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one teacher head, not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2025
Admission routes2
Has abstractyes

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