Comparison of alemtuzumab and ATG-based conditioning in adult recipients of allogeneic hematopoietic cell transplantation (HCT) for severe aplastic anemia: A retrospective study
Bibliographic record
Abstract
Abstract Background The optimal in-vivo T-cell depleting agent for allogeneic hematopoietic cell transplantation (HCT) for patients with severe aplastic anemia (SAA) remains unclear. Current guidelines endorse the use of either rabbit anti-thymocyte globulin (ATG) or alemtuzumab, with selection guided by institutional experience and availability. We aimed to compare clinical outcomes in adults with acquired SAA undergoing HCT from matched sibling (MSD) or unrelated donors (MUD) using either ATG-based conditioning or a historical cohort treated with alemtuzumab-based regimens. Methods A retrospective analysis of patients with acquired SAA who underwent HCT at our center between May 2010 and 2024 was conducted. The ATG-based regimen consisted of intravenous (IV) fludarabine 30 mg/m²/day from day −5 to −2, cyclophosphamide 60 mg/kg/day on days −5 and −4, and rabbit anti-thymocyte globulin (Thymoglobulin) at a total dose of 4.5 mg/kg, administered over days -3 to -1. The alemtuzumab-based regimen included identical doses of fludarabine and cyclophosphamide, with alemtuzumab at a total dose of 60 mg, administered over days−8 to −4. For patients receiving MUD grafts, low-dose total body irradiation (TBI) at 200 cGy was added on day −1. Results A total of 62 patients (median age: 30 years (IQR: 25 – 48) underwent HCT during the study period. MSD were used in 34 patients (55%). Alemtuzumab-based conditioning was used in 20 patients (32%), while the remaining received ATG. Median follow-up of the entire cohort was 59 months (range: 48–72), significantly longer in the alemtuzumab group [134 months (99–156) vs. 47 months (21–49), p<0.01]. Bone marrow (BM) grafts were used in 39 patients (63%), with a higher proportion in the alemtuzumab group (81% vs. 53%, p=0.02). The median nucleated cell dose was 3.3 (2.2–3.9) ×10⁸/kg for BM grafts, and 7 (5 – 8) x 106/kg for peripheral blood stem cell grafts, and was similar between groups. Median time to neutrophil engraftment was 19 days (range: 16–32) with alemtuzumab and 17 days (15–21) with ATG (p=0.34). Platelet engraftment occurred earlier in the alemtuzumab group (median 12 days vs. 20 days with ATG, p=0.03). Complete donor chimerism at day +30 was observed in 42 patients, with no difference between the groups. Primary graft failure occurred in 10 patients, including 6 in the alemtuzumab group. The cumulative incidence of primary graft failure was 13% (95% CI: 3–31) with alemtuzumab and 8% (95% CI: 2–19) with ATG (p=0.12). At day +100, rates of acute grade II–IV graft versus host disease (aGvHD) were 23% with alemtuzumab and 15% with ATG (p=0.63); grade III–IV aGvHD occurred in 9% vs. 1%, respectively (p=0.27). At two years, the incidence of chronic GvHD (all grades) was 4.5% (1–19) with alemtuzumab and 14% (5–27) with ATG (p=0.57). Eight patients died during follow-up: 2 from primary graft failure, 1 from aGvHD, and 5 from infections, with no significant differences in causes of death between the two groups. Three-year event-free survival (EFS) was 64% (40–78) and 70% (53–83), and overall survival (OS) was 91% (68–97) and 85% (68–93) in the alemtuzumab and ATG groups, respectively (p=0.59 and p=0.43). On multivariable analysis, alemtuzumab use was associated with faster platelet engraftment, with no significant impact on rates of acute or chronic GvHD, primary graft failure, EFS, or OS. Conclusion Alemtuzumab-based conditioning was associated with faster platelet engraftment compared to ATG, with comparable rates of graft failure, GvHD, EFS, and OS between the two groups. Our study suggests that either agent is a reasonable option, with choice guided by availability and institutional experience.
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.002 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.001 | 0.001 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".