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Record W4417152316 · doi:10.1038/s41467-025-65993-5

A resource to empirically establish drug exposure records directly from untargeted metabolomics data

2025· article· en· W4417152316 on OpenAlexaff
Haoqi Nina Zhao, Kine Eide Kvitne, Corinna Brungs, Siddharth Mohan, Vincent Charron‐Lamoureux, Wout Bittremieux, Runbang Tang, Robin Schmid, Santosh Lamichhane, Shipei Xing, Yasin El Abiead, Mohammad Sobhan Sheikh Andalibi, Helena Mannochio-Russo, Madison Ambre, Nicole E. Avalon, MacKenzie Bryant, Lindsey A. Burnett, Andrés Mauricio Caraballo‐Rodríguez, Martín Casas Maya, Loryn Chin, Lluís Corominas, Ronald J. Ellis, Donald Franklin, Sagan Girod, Paulo Wender Portal Gomes, Lauren Hansen, Robert K. Heaton, Jennifer E. Iudicello, Alan K. Jarmusch, Lora Khatib, Scott Letendre, Sarolt Magyari, Daniel McDonald, Ipsita Mohanty, Andrés Cumsille, David J. Moore, Prajit Rajkumar, Dylan H. Ross, Harshada Sapre, Mohammad Reza Zare Shahneh, Rubén Gil‐Solsona, Sydney P. Thomas, Caitlin Tribelhorn, Helena M. Tubb, Corinn Walker, Crystal X. Wang, Jasmine Zemlin, Simone Zuffa, David S. Wishart, Pablo Gago-Ferrero, Rima Kaddurah‐Daouk, Mingxun Wang, Manuela Raffatellu, Karsten Zengler, Tomáš Pluskal, Libin Xu, Rob Knight, Shirley M. Tsunoda, Pieter C. Dorrestein

Bibliographic record

VenueNature Communications · 2025
Typearticle
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicMetabolomics and Mass Spectrometry Studies
Canadian institutionsUniversity of Alberta
FundersEunice Kennedy Shriver National Institute of Child Health and Human DevelopmentNational Center for Complementary and Integrative HealthNational Institute of Environmental Health SciencesNational Institute of Diabetes and Digestive and Kidney DiseasesNational Institute of Allergy and Infectious DiseasesNational Institute of Mental HealthNational Institute on AgingNational Institutes of Health
KeywordsDrugResource (disambiguation)Drug discoveryMetabolomicsDrug developmentHuman immunodeficiency virus (HIV)OntologyAutomatic summarization

Abstract

fetched live from OpenAlex

Despite extensive efforts, extracting medication exposure information from clinical records remains challenging. To complement this approach, here we show the Global Natural Product Social Molecular Networking (GNPS) Drug Library, a tandem mass spectrometry (MS/MS) based resource designed for drug screening with untargeted metabolomics. This resource integrates MS/MS references of drugs and their metabolites/analogs with standardized vocabularies on their exposure sources, pharmacologic classes, therapeutic indications, and mechanisms of action. It enables direct analysis of drug exposure and metabolism from untargeted metabolomics data, supporting flexible summarization at multiple ontology levels to align with different research goals. We demonstrate its application by stratifying participants in a human immunodeficiency virus (HIV) cohort based on detected drug exposures. We uncover drug-associated alterations in microbiota-derived N-acyl lipids that are not captured when stratifying by self-reported medication use. Overall, GNPS Drug Library provides a scalable resource for empirical drug screening in clinical, nutritional, environmental, and other research disciplines, facilitating insights into the ecological and health consequences of drug exposures. While not intended for immediate clinical decision-making, it supports data-driven exploration of drug exposures where traditional records are limited or unreliable.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.007
metaresearch head score (Gemma)0.033
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: none
GenreCandidate signal: Methods · Consensus signal: none
Teacher disagreement score0.014
Threshold uncertainty score0.047

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0070.033
Meta-epidemiology (narrow)0.0010.001
Meta-epidemiology (broad)0.0010.001
Bibliometrics0.0090.007
Science and technology studies0.0010.000
Scholarly communication0.0020.003
Open science0.0020.004
Research integrity0.0010.001
Insufficient payload (model declined to judge)0.0140.008

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.020
GPT teacher head0.315
Teacher spread0.296 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreMethods

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations3
Published2025
Admission routes1
Has abstractyes

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Same venueNature Communications→Same topicMetabolomics and Mass Spectrometry Studies→French-language works237,207→