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Record W4417274105 · doi:10.1021/acs.jnatprod.5c01104

Discovery of Fusadapamides, Accessory Chromosome-Associated Metabolites Incorporating <scp>l</scp> -2,3-Diaminopropionic Acid in <i>Fusarium poae</i>

2025· article· en· W4417274105 on OpenAlexafffund
Thomas E. Witte, Linda J. Harris, L. A. PAQUETTE, Anne Hermans, Amanda Sproule, Anne Johnston, Michael G. Darnowski, Whynn Bosnich, Danielle Schneiderman, Xiben Wang, Benjamin A G Beavington, Izhar U. H. Khan, Christopher N. Boddy, David P. Overy

Bibliographic record

VenueJournal of Natural Products · 2025
Typearticle
Languageen
FieldMedicine
TopicMicrobial Natural Products and Biosynthesis
Canadian institutionsUniversity of OttawaAgriculture and Agri-Food CanadaInternational Development Research Centre
FundersAgriculture and Agri-Food CanadaNatural Sciences and Engineering Research Council of Canada
KeywordsMetabolomicsNatural productGene clusterBiosynthesisGenomeTripeptideGeneSecondary metabolism

Abstract

fetched live from OpenAlex

High Resolution Image Download MS PowerPoint Slide Genome mining of fungal plant pathogens has uncovered biosynthetic gene clusters encoded on lineage-specific accessory chromosomes, revealing untapped potential for novel natural product discovery by metabolomic assessment of fungal populations. In Fusarium poae, a species contributing to Fusarium head blight on cereals, whole-genome sequencing and comparative metabolomics identified an accessory chromosome-associated biosynthetic gene cluster responsible for the production of a novel family of secondary metabolites, the fusadapamides. These linear tripeptides contain l -2,3-diaminopropionic acid ( l -Dap), a rare nonproteinogenic amino acid not previously reported in fungi. Biochemical and genetic analyses revealed that F. poae synthesizes l -Dap via an accessory chromosome-encoded two-gene module that uniquely utilizes l -alanine as a substrate, diverging from known bacterial and plant l -Dap biosynthesis pathways. While fusadapamide production appears limited within Fusarium, homologous l -Dap biosynthetic modules were identified across diverse ascomycetes, suggesting a broader role in fungal secondary metabolism. This study highlights the power of using untargeted metabolomics at population-scale to uncover accessory chromosome-linked biosynthetic innovations and expands our understanding of fungal natural product biosynthesis.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.001
Threshold uncertainty score0.002

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0010.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.011
GPT teacher head0.249
Teacher spread0.238 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2025
Admission routes2
Has abstractyes

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