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Design of experiments (DOE) analysis of the effects of environmental conditions on bloodstain degradation using spectroscopic methods

2025· article· en· W4417524168 on OpenAlexafffund
Laurianne Huard, Frank Crispino, Cyril Muehlethaler

Bibliographic record

VenueForensic Science International · 2025
Typearticle
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicForensic and Genetic Research
Canadian institutionsUniversité du Québec à MontréalUniversité du Québec à Trois-Rivières
FundersNatural Sciences and Engineering Research Council of Canada
KeywordsHyperspectral imagingRaman spectroscopyDegradation (telecommunications)SpectroscopyHumidityRelative humidity

Abstract

fetched live from OpenAlex

Blood is one of the most common types of trace found at crime scenes. However, from a forensic point of view, the potential of blood traces is still not fully exploited, as there is as yet no reliable technique for dating blood traces found at scenes. The same difficulties are cited in the literature for published research on blood traces: the mechanisms of blood degradation are known and validated, but traces found at scenes are not controlled samples. Traces therefore need to be evaluated in the light of this uncertainty and the factors that can influence the blood trace dating model. In the course of this project, we assessed the significance of the environmental effects of temperature (10-40°C), humidity (25-75 %) and radiation (none and maximum) using three different spectroscopic techniques: Raman spectroscopy, MicroNIR spectroscopy and hyperspectral imaging. The use of a climatic chamber with the addition of LED lamps (daylight and UV) enabled parameters to be controlled during a 3-day aging period for each sample. By means of a 2-level experimental screening design of the three factors, we were able to observe complementarity between the methods used. Raman spectroscopy highlighted the influence of temperature, MicroNIR spectroscopy provided information on the influence of temperature and relative humidity, and hyperspectral imaging demonstrated the influence of temperature and the presence of radiation. These results provide a better understanding of the factors that cause the blood degradation model to deviate, enabling us to develop a more comprehensive model of these factors.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.052
Threshold uncertainty score0.275

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.001
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.020
GPT teacher head0.388
Teacher spread0.368 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one teacher head, not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2025
Admission routes2
Has abstractyes

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