Contraction Study of a Single Cardiac Muscle Cell in a Microfluidic Chip
Bibliographic record
Abstract
This chapter introduces a microfluidic method to study the contraction of a single cardiac muscle cell (cardiomyocyte). This method integrates single-cell selection, cell retention, dye loading, chemical stimulation, and fluorescence measurement for intracellular calcium on one microfluidic chip. Before single-cell experiments, the bonded chip was modified in order to make the channel deep enough to accommodate a large, single cardiomyocyte. After the modification, a single heart muscle cell could be selected and retained at a cell retention structure. Fluo-4 AM was loaded in the cell for the measurement of intracellular calcium ion concentration in the cell. Subsequently, caffeine was introduced into the chamber to induce the contraction of the cardiomyocyte. During contraction, fluorescence measurement was used to monitor the intracellular calcium level,and an optical imaging system was used to monitor the shape to confirm the contraction. The resting [Ca2+]i of cardiomyocyte was determined and was consistent with the value of approx 100 nM in the literature.
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".