Suppression of phenylephrine contraction by AMPK inhibition in isolated carotid artery from normotensive WKY and hypertensive SHR rats
Bibliographic record
Abstract
AMP‐activated protein kinase (AMPK) is emerging as an important regulator of vascular function and has differential effects on vascular tone in arteries from normotensive Wistar Kyoto (WKY) and spontaneously hypertensive (SHR) rats. The current study aims to determine: if AMPK modulates the vasocontraction response to phenylephrine (PE); if AMPK effects are mediated by a downstream ROCK effect; and, if hypertension alters the regulatory effects of AMPK. Isolated common carotid artery (CCA) segments (denuded of endothelium) from WKY and SHR were used to determine vasomotor dose‐responses to PE (10 −8 –10 −4 M) after control (CON) incubation, or incubation with: AMPK inhibitor Compound C (CC; 20 μM); ROCK inhibitor Y26732 (1 μM); or, CC+Y26732. PE contraction was suppressed in SHR vs WKY CON (EC 50 55±6 vs 17 ± 4 nM, P <0.05). CC or Y26732 each suppressed PE contraction (increased EC 50 vs respective CON) in CCA of each of WKY and SHR. The CC + Y26732 effect was greater than either individual drug effect in both WKY and SHR, but was not completely additive. While the absolute values of EC 50 were higher in SHR vs WKY for each condition, the effects of CC and Y26732 on PE EC 50 were relatively (to respective CON) greater in WKY vs SHR. Results suggest that AMPK may contribute to modulating PE contraction in denuded CCA via Rock‐dependent and –independent mechanisms, and that hypertension suppresses this effect of AMPK. Supported by NSERC
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.001 | 0.001 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.002 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".