The Effect of WDR1 and a Spliced Isoform (WDRΔ35) on Cell Migration in Mammalian Cells
Bibliographic record
Abstract
WDR1 is a highly conserved regulator of the actin cytoskeleton in eukaryotes. A novel splice variant of WDR1 has been discovered, WDRΔ35, which lacks exons 3-5. To determine if functional differences exist between these two isoforms and whether perturbation of WDR1/WDRΔ35 levels affects the rate of cell migration, they were overexpressed as GFP fusion proteins in HEK293 cells and the rates of cell migration were quantified. Overexpression of WDR1/WDRΔ35 caused a significant decrease in cell migration compared to the GFP control. The ratio of G/F-actin was measured upon WDR1/WDRΔ35 overexpression and it was found to be significantly higher for GFP-WDR/ WDRΔ35 transfected cells compared to GFP alone which may indicate higher actin turnover rates in these cells. Site directed mutagenesis generated several mutants for WDR1/ WDRΔ35 which indicated that specific residues (e.g. WDRR17G, WDRΔ35H48Q and WDRΔ35G204E) could alter the ratio of G/F-actin in cells, suggesting an important structural role in WDR1 function.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".