The roles of actin tyrosine phosphorylation and cellulose during dormancy and germination of various Dictyostelium discoideum mutants.
Bibliographic record
Abstract
Actin tyrosine phosphorylation plays a critical role in maintaining spore dormancy and spore viability. In the slime mold, Dictyostelium discoideum, several strains were explored for spore morphology, actin tyrosine phosphorylation levels, and the presence of cellulose in the spore coat layers of the cell walls in both dormant and germinating spores. On average the actin tyrosine phosphorylation levels were maximized on the fourth to sixth day and there after slowly decreased as the spores reached the age of 14 days. Actin tyrosine phosphorylation patterns for NC4, SG1, SG2, Ax3, and RegA - show an overall decrease for the 15°C grown cultures in comparison to the RT grown cultures. Cellulose studies revealed that cellulose: was present in the middle layer of the spore coat cell wall; was present in dormant, swelling, and emerging spores, but absent in nascent amoebae; demonstrated that there is a certain location within the spore coat cell wall that is a common tearing or splitting point during the natural process (germination), but not for the mechanical process (glass beads); and was present throughout the spore. (Abstract shortened by UMI.)Dept. of Biological Sciences. Paper copy at Leddy Library: Theses & Major Papers - Basement, West Bldg. / Call Number: Thesis2001 .M38. Source: Masters Abstracts International, Volume: 40-03, page: 0653. Adviser: David A. Cotter. Thesis (M.Sc.)--University of Windsor (Canada), 2001.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".