Bibliographic record
Abstract
根据小麦和长穗偃麦草的液泡膜Na^＋／H^＋逆转运蛋白基因（TaNHX1、TeNHX1）全长序列设计引物，通过RT-PCR直接扩增的方法从毛偃麦草（Elytrigia trichophora L．）中克隆到了Na^＋／H^＋逆转运蛋白基因，命名为EtNHX1（Accession numeber：EU876834）。EtNHX1最大开放阅读框为1641bp，编码含有546个氨基酸残基、分子量为59．8kD的蛋白，预测等电点8．0。EtNHX1含有39个碱性氨基酸，37个酸性氨基酸，256个疏水氨基酸及128个极性氨基酸。二级结构预测表明该蛋白含约47％的α-螺旋、20％的延伸链、4．5％的β-转角和28％的不规则卷曲。亲疏水性分析显示，EtNHX1含有12个连续的疏水片断，其中10个可能构成跨膜螺旋。序列分析显示，EtNHX1与小麦（Triticum aestivumL．）、中间偃麦草（Thinopyrum intermedium L．）、长穗偃麦草（Elytrigia elongate L．）、水稻（Oryza sativa L．）、角果碱蓬（Suaeda corniculata L．）、小盐芥（Thellungiella halophila L．）等植物的液泡膜Na^＋／H^＋逆向转运蛋白高度同源，序列相似性分别为98％、98％、96％、85％、68％和67％。序列比对结果以及进化树分析均表明EtNHX1应为定位于毛偃麦草液胞膜上的Na^＋/H^＋逆向转运蛋白。
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.001 | 0.000 |
| Insufficient payload (model declined to judge) | 0.006 | 0.002 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; both teacher heads agree on what is shown here.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".