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Record W6893307977 · doi:10.5281/zenodo.15160495

Table 1 in West Palaearctic species of Euura Newman, 1837 (Hymenoptera, Tenthredinidae)

2025· dataset· en· W6893307977 on OpenAlexaff

Bibliographic record

VenueZenodo (CERN European Organization for Nuclear Research) · 2025
Typedataset
Languageen
FieldEngineering
TopicAnalog and Mixed-Signal Circuit Design
Canadian institutionsUniversity of Guelph
Fundersnot available
KeywordsAmpliconPrimer (cosmetics)Multiplex polymerase chain reactionTable (database)spliceMultiplex

Abstract

fetched live from OpenAlex

Table 1. Amplicons, primers and multiplex PCR mixes. The listed genes were amplified in two (mixes 1–2) or three (mixes 1–3) different multiplex PCR reactions. In a few species, some of the introns are missing in PGD, TPI, or EF1a F2. AmpliconPCR mixPrimers (forward + reverse)Concentration in the primer mix (pmol/µl)Amplicon length (bp)# introns 1COI1SymF5 +A2590210870PGD1PGD326F + PGD_hym_intRb3.5751–1400+1TPI1TPI35F + TPI706Rv24~720–8502EF1a F11EF1a47F + EF1a1282R512340EF1a F2 21EF1a47F + EF1a1282R~1600–2400+3ITS 31ITS-p5 + ITS-4unR11~600–1900+2NaK2NaK_263Fv2 + NaK_1918Rv2316540HSP90 h12HSP90_59F + HSP90_1843Rv25.517800HSP90 h22HSP90_59F + HSP90_1843Rv2~1900–3500+2POL22POL2_797F + POL2_2569R417710EF1a F2 52EF1a167F + EF1a1213R + EF1a1213Ra4~1300–2200+2TRRAP2 6TRRAP_833F + TRRAP_2620R51774–17860HSP90 h2 73HSP90b_83F + HSP90_1843Rv24~1900–3500+2POL23POL2_104Fv2 + POL2_2569R5~2500–2600 81TRRAP3TRRAP_833F + TRRAP_3784R52938–29500 1 Number of introns in the amplicon region. 2 The primers match both copies of the EF1a, but amplification of EF1a F2 usually does not work because it is much longer than F1 copy due to introns. 3 The plant specific ITS primers were included in the mix 1 only occasionally to identify the hosts of larvae. Amplicon length in angiosperms is usually around 650–750 bp. 4 The primers match both copies of the HSP90, but amplification of HSP90 h2 usually does not work because it is much longer than h1copy due to introns. 5 Two EF1a F2 specific (do not match the F1 copy at the 3’ end) reverse primers are included due to variability at the 3’ end. 6 This TRRAP amplicon was included in the mix 2 later in the study and mix 3 was then abandoned. 7 The forward primer is specific to h2 copy (does not match the h1 copy at the 3’ end). 8 The intron length varies little in most Nematinae, but in some Hoplocampa it can be much longer, resulting in an amplicon length of more than 3300 bp.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesInsufficient payload (model declined to judge)
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Not applicable · Consensus signal: none
GenreCandidate signal: Dataset · Consensus signal: none
Teacher disagreement score0.957
Threshold uncertainty score0.142

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0010.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0010.002
Science and technology studies0.0010.000
Scholarly communication0.0000.001
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0430.014

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.023
GPT teacher head0.218
Teacher spread0.195 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

Study designNot applicable
Domainnot available
GenreDataset

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2025
Admission routes1
Has abstractyes

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