Additional file 1 of The DNMT1 inhibitor GSK-3484862 mediates global demethylation in murine embryonic stem cells
Bibliographic record
Abstract
Additional file 1: Figure S1. A. Western blot for DNMT1 as well as H3 loading control in WT and Dnmt1-/- 3a−/− 3b−/− mESCs. Note loss of DNMT1 in the triple knockout cells, indicating antibody specificity. B. Western blot for DNMT1 as well as H3 loading control in WT mESCs and mESCs treated with the indicated concentration of GSK-3484862 for four days. Quantitation of the DNMT1 band, relative to the H3 loading control, is indicated. Figure S2. A., B. DNA methylation level of 10-kb regions of the genome are indicated, with each region plotted as a single point. C. A violin plot showing the overall methylation level of the 812 full length IAP-Ez elements (> 6 kb long) in the cell types indicated. Each IAP-Ez element is represented as a single point on the violin plot.
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.002 | 0.015 |
| Meta-epidemiology (narrow) | 0.001 | 0.001 |
| Meta-epidemiology (broad) | 0.002 | 0.001 |
| Bibliometrics | 0.002 | 0.003 |
| Science and technology studies | 0.001 | 0.000 |
| Scholarly communication | 0.002 | 0.002 |
| Open science | 0.002 | 0.001 |
| Research integrity | 0.001 | 0.001 |
| Insufficient payload (model declined to judge) | 0.906 | 0.308 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".